174844-42-9 Usage
Description
2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)aMino]-2-deoxy-D-glucose, also known as 2-NBDG, is a fluorescent glucose analog that serves as a valuable tool in cell biology and metabolic studies. It is a non-metabolizable derivative of glucose, which allows it to be taken up by cells through glucose transporters and detected using fluorescence microscopy or flow cytometry. The fluorescence properties of 2-NBDG make it an essential marker for studying glucose transport and metabolism in various cell types, such as cancer cells, adipocytes, and muscle cells.
Uses
Used in Cancer Research:
2-NBDG is used as a glucose uptake marker in cancer research for studying the altered metabolism of cancer cells. It helps in understanding the Warburg effect, where cancer cells exhibit increased glucose uptake and glycolysis even in the presence of oxygen. This knowledge is crucial for developing targeted therapies and diagnostic tools for cancer.
Used in Diabetes Studies:
In diabetes research, 2-NBDG is employed as a tool to investigate glucose uptake and metabolism in cells, particularly in the context of insulin resistance and pancreatic β-cell function. This helps in understanding the molecular mechanisms underlying diabetes and contributes to the development of potential therapeutic interventions.
Used in Exercise Physiology:
2-NBDG is used as a glucose uptake marker in exercise physiology to study the effects of physical activity on glucose metabolism in muscle cells. This research aids in understanding the role of exercise in maintaining glucose homeostasis and developing strategies for managing and preventing metabolic disorders.
Used in Cell Biology Research:
In cell biology, 2-NBDG is utilized as a fluorescent probe to study glucose transport mechanisms, intracellular glucose metabolism, and the regulation of glucose transporters. This research provides insights into the cellular processes and contributes to the understanding of various physiological and pathological conditions related to glucose metabolism.
Used in Drug Discovery and Development:
2-NBDG is employed as a tool in drug discovery and development for identifying and evaluating potential therapeutic agents that target glucose metabolism and transport. This helps in the development of new drugs for treating metabolic disorders, such as diabetes and obesity, and other conditions associated with altered glucose metabolism.
Check Digit Verification of cas no
The CAS Registry Mumber 174844-42-9 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,7,4,8,4 and 4 respectively; the second part has 2 digits, 4 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 174844-42:
(8*1)+(7*7)+(6*4)+(5*8)+(4*4)+(3*4)+(2*4)+(1*2)=159
159 % 10 = 9
So 174844-42-9 is a valid CAS Registry Number.
174844-42-9Relevant articles and documents
Improved synthesis of 4-amino-7-nitrobenz-2,1,3-oxadiazoles using NBD fluoride (NBD-F)
Jung, Michael E.,Dong, Timothy A.,Cai, Xiaolu
, p. 2533 - 2535 (2011/06/21)
The 7-nitrobenz-2,1,3-oxadiazole (NBD) unit is a highly useful fluorescent tag with wide application in biology. Installation of the NBD group typically proceeds via the SNAr reaction between an amine and an NBD halide. Herein, we demonstrate that NBD-F 1 results in significantly higher yields than NBD-Cl 2, and that triethylamine in dimethylformamide at 23 °C overnight is a broadly applicable set of conditions for this reaction. In particular, the highly useful fluorescent carbohydrate 2-NBD-glucosamine (2-NBDG, 3) can now be prepared in 75% yield with NBD-F as compared to 12% with NBD-Cl.
