178925-55-8

Basic information

• Product Name: WST-5
• Synonyms: WST-5;2,2'-Dibenzothiazolyl-5,5'-bis[4-di(2-sulfoethyl)carbamoylphenyl]-3,3'-(3,3'-dimethoxy-4,4'-biphenylene)ditetrazolium,disodiumsalt;2,2''-Dibenzothiazolyl-5,5''-bis-[4-bis-(2-sulfoethyl)-carbamoylphenyl]-3,3''-;2,2''-DIBENZOTHIAZOLYL-5,5''-BIS(4-DI(2-SULFOETHYL)CARBAMOYLPHENYL)-3,3''-(3,3''-DIMETHOXY-4,4''-BIPHENYL;2,2'-Dibenzothiazolyl-5,5'-bis(4-di(2-sulfoethyl)carbamoylphenyl;GLT005;2,2'-(3,3'-DiMethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(2-benzothiazolyl)-5-[4-[[bis(2-sulfoethyl)aMino]carbonyl]phenyl]-2H-tetrazoliuM Bis(inner salt) SodiuM Salt;2,2'-(3,3'-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(2-benzothiazolyl)-5-[4-[[bis(2-sulfoethyl)amino]carbonyl]phenyl]-2H-tetrazolium bis(inner salt) disodium salt
• CAS NO: 178925-55-8
• Molecular Formula: C52H44N12O16S6.2Na
• Molecular Weight: 1331.36
• Product Categories: Aromatics;Heterocycles;Intermediates & Fine Chemicals;Labeling and Diagnostics Reagents;Pharmaceuticals
• Mol File: 178925-55-8.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• CAS DataBase Reference: WST-5(CAS DataBase Reference)
• NIST Chemistry Reference: WST-5(178925-55-8)
• EPA Substance Registry System: WST-5(178925-55-8)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36
• Hazardous Substances Data: 178925-55-8(Hazardous Substances Data)

Usage

Description

WST-5 is a highly sensitive tetrazolium reagent that is utilized in various applications due to its ability to produce a water-soluble diformazan (blue color) in NADH. It is characterized by its formazan l max at 580nm and an extinction coefficient (e) of 2.7 x 10^4.

Uses

Used in Cell Proliferation and Viability Assays:
WST-5 is used as a cell proliferation and viability assay reagent for its ability to measure the activity of cellular enzymes, such as dehydrogenases, which are essential for cellular respiration and energy production. The blue-colored formazan produced by WST-5 can be easily quantified, allowing researchers to assess the health and metabolic activity of cells in various experimental conditions.
Used in Cytotoxicity Studies:
In the pharmaceutical and biotechnology industries, WST-5 is used as a cytotoxicity assay reagent to evaluate the potential toxic effects of drugs, chemicals, or other substances on cells. By measuring the reduction in cellular metabolic activity, researchers can determine the concentration of a substance that causes a specific level of cytotoxicity, which is crucial for drug safety assessment and development.
Used in Immunoassays:
WST-5 is also employed in immunoassays, particularly in enzyme-linked immunosorbent assays (ELISAs), as a substrate for colorimetric detection of specific antibodies or antigens. The blue-colored formazan produced by WST-5 upon enzymatic conversion serves as a visual or quantifiable signal, enabling the detection and quantification of target molecules in various diagnostic and research applications.
Used in Research and Development:
In the field of life sciences and biomedical research, WST-5 is used as a versatile tool for studying various cellular processes, such as cell growth, differentiation, and apoptosis. Its high sensitivity and ease of use make it an invaluable resource for researchers investigating the mechanisms underlying various diseases and for developing new therapeutic strategies.