18422-05-4 Usage
Description
Adenosine 5'-monophosphate monohydrate (5'-AMP) is a white crystalline powder that serves as an essential nucleotide in various biological processes. It is a key component in the synthesis of adenosine triphosphate (ATP), the primary energy currency of cells, and plays a crucial role in cellular energy metabolism.
Uses
1. Used in Pharmaceutical Industry:
Adenosine 5'-monophosphate monohydrate is used as a reagent for the synthesis of adenosine-5'-phosphoimidazolidean, an activator of phosphorylase b. This activator is involved in the polymerization reaction for the synthesis of branched polysaccharides, which are vital for various cellular functions.
2. Used in Biochemical Research:
5'-AMP is used as an inhibitor of endogenous nicotinamide mononucleotide adenylyltransferase (NMNAT) activity. This enzyme is responsible for converting nicotinamide mononucleotide (NMN) to nicotinamide adenine dinucleotide (NAD+), a coenzyme that plays a critical role in cellular redox reactions and energy metabolism.
3. Used in Nutritional Supplements:
Adenosine 5'-monophosphate monohydrate is used as a nutrient in the formulation of dietary supplements. It is believed to enhance energy production, support immune function, and improve overall health.
4. Used in Cardiology:
5'-AMP is used as a Ca++ channel blocker, which can help regulate the flow of calcium ions into cardiac muscle cells. This property makes it useful in the treatment of certain heart conditions, such as arrhythmias and hypertension.
Pharmacodynamics
Adenosine monophosphate, also known as 5'-adenylic acid and abbreviated AMP, is a nucleotide that is found in RNA. It is an ester of phosphoric acid with the nucleoside adenosine. AMP consists of the phosphate group, the pentose sugar ribose, and the nucleobase adenine. AMP is used as a dietary supplement to boost immune activity, and is also used as a substitute sweetener to aid in the maintenance of a low-calorie diet.
Biochem/physiol Actions
Adenosine 5′-monophosphate (5′-AMP) has many uses in nature. 5′-AMP is an activator of a class of protein kinases known as AMP-activated protein kinase (AMPK). AMP inhibits dephosphorylation of AMPK and promotes phosphorylation of AMPK by upstream kinases.
Purification Methods
The acid has been recrystallised from H2O (fine needles) and is freely soluble in boiling H2O. It crystallises also from H2O on addition of acetone. Alternatively purify it by chromatography on Dowex 1 (in formate form), eluting with 0.25M formic acid. It is then adsorbed onto charcoal (which had been boiled for 15minutes with M HCl, washed free of chloride and dried at 100o) and recovered by stirring three times with isoamyl alcohol/H2O (1:9 v/v). The aqueous layer from the combined extracts is evaporated to dryness under reduced pressure, and the product is crystallised twice from hot H2O. [Morrison & Doherty Biochem J 79 433 1961]. It has max 259nm ( 15,400) in H2O at pH 7.0. [Alberty et al. J Biol Chem 193 425 1951, Martell & Schwarzenbach Helv Chim Acta 39 653 1956]. The acridinium salt has m 208o [Baddiley & Todd J Chem Soc 648 1947, Pettit Synthetic Nucleotides, van Nostrand-Reinhold, NY, Vol 1 252 1972, NMR: Sarma et al. J Am Chem Soc 96 7337 1974, Norton et al. J Am Chem Soc 98 1007 1976, IR of diNa salt: Miles Biochem Biophys Acta 27 324 1958]. [Beilstein 26 III/IV 3615.]
Check Digit Verification of cas no
The CAS Registry Mumber 18422-05-4 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,8,4,2 and 2 respectively; the second part has 2 digits, 0 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 18422-05:
(7*1)+(6*8)+(5*4)+(4*2)+(3*2)+(2*0)+(1*5)=94
94 % 10 = 4
So 18422-05-4 is a valid CAS Registry Number.
