201853-23-8 Usage
Description
H-LYS-AMC ACOH, also known as L-Lysine 7-amido-4-methylcoumarin acetate salt, is a synthetic substrate commonly used in biochemical research. It is designed to mimic the structure of peptide bonds found in proteins, allowing for the study of enzyme activity and function.
Uses
Used in Enzyme Activity Assays:
H-LYS-AMC ACOH is used as a substrate for aminopeptidase B, an enzyme involved in the breakdown of proteins. Upon cleavage by aminopeptidase B, the substrate produces a blue fluorescent solution, which can be measured to assess enzyme activity. This application is particularly useful in studying the function and regulation of aminopeptidases, as well as in the development of potential inhibitors or activators of these enzymes.
Used in Analytical Chemistry:
H-LYS-AMC ACOH is also used as an analyte in analytical studies, specifically for the enhanced extraction of amino compounds. In an aqueous two-phase system, dicyclohexyl-18-crown-6 is employed as a ligand to selectively bind and extract amino compounds, including H-LYS-AMC ACOH, from complex mixtures. This technique is valuable for the purification and analysis of amino acids and peptides in various industries, such as pharmaceuticals, food, and cosmetics.
Check Digit Verification of cas no
The CAS Registry Mumber 201853-23-8 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,0,1,8,5 and 3 respectively; the second part has 2 digits, 2 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 201853-23:
(8*2)+(7*0)+(6*1)+(5*8)+(4*5)+(3*3)+(2*2)+(1*3)=98
98 % 10 = 8
So 201853-23-8 is a valid CAS Registry Number.
