2022-78-8Relevant articles and documents
Cloned cytosine deaminase gene expression of Bifidobacterium longum and application to enzyme/pro-drug therapy of hypoxic solid tumors.
Nakamura, Toshiyuki,Sasaki, Takayuki,Fujimori, Minoru,Yazawa, Kazuyuki,Kano, Yasunobu,Amano, Jun,Taniguchi, Shun'ichiro
, p. 2362 - 2366 (2002)
Bifidobacterium longum is a nonpathogenic anaerobic bacterium among normal bacterial flora. Recently, it was reported that B. longum accumulated in hypoxic solid tumors. The gene of interest was expressed in transfected B. longum by the shuttle vector pBLES100 in solid tumors. In this report, we constructed pBLES100-S-eCD, which included the cytosine deaminase gene. We confirmed by western blotting that transfected B. longum produced cytosine deaminase. In addition, transfected B. longum produced cytosine deaminase that converted 5-fluorocytosine into 5-fluorouracil. B. longum could be useful for enzyme/pro-drug therapy of hypoxic solid tumors.
Antitumor Properties of 2(1H)-Pyrimidinone Riboside (Zebularine) and Its Fluorinated Analogues
Driscoll, John S.,Marquez, Victor E.,Plowman, Jacqueline,Liu, Paul S.,Kelley, James A.,Barchi, Joseph J.
, p. 3280 - 3284 (2007/10/02)
2(1H)-Pyrimidinone riboside (zebularine, 1b) and its 5-fluoro (6b) and 2'-ara-fluoro (7b) analogues have been synthesized and evaluated in vivo as antitumor agents.Zebularine provides increase in life span (ILS) values of ca. 70percent against intraperito