2056-98-6

Basic information

• Product Name: 2'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE LITHIUM SALT
• Synonyms: 2'-DEOXYCYTIDINE-5'-TRIPHOSPHORIC ACID, LITHIUM;2'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE LITHIUM SALT;DCTP, LI;DCTP LI-SALT;Cytidine 5'-(tetrahydrogen triphosphate), 2'-deoxy-;2'-deoxycytidine triphosphate;[[[5-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxyphosphonic acid;2''-DEOXYCYTIDINE 5''-TRIPHOSPHATE (DCTP)
• CAS NO: 2056-98-6
• Molecular Formula: C₉H₁₆N₃O₁₃P₃
• Molecular Weight: 473.09
• EINECS: 218-153-9
• Product Categories: Nucleic acids
• Mol File: 2056-98-6.mol
• Article Data: 7

Chemical Properties

• Boiling Point: 811.5 °C at 760 mmHg
• Flash Point: 444.6 °C
• Appearance: /Stabilized Aqueous Solution
• Density: 2.38 g/cm³
• Storage Temp.: Sealed in dry,Room Temperature
• PKA: 0.80±0.50(Predicted)
• CAS DataBase Reference: 2'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE LITHIUM SALT(CAS DataBase Reference)
• NIST Chemistry Reference: 2'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE LITHIUM SALT(2056-98-6)
• EPA Substance Registry System: 2'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE LITHIUM SALT(2056-98-6)

Safety Data

• Hazardous Substances Data: 2056-98-6(Hazardous Substances Data)

Usage

General Description

2'-Deoxy-cytidine-5'-triphosphate lithium salt is a chemical compound that belongs to the class of nucleoside triphosphates and is commonly used in molecular biology and biochemistry research. It is an important building block for the synthesis of DNA and RNA and is crucial for DNA replication, repair, and recombination. 2'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE LITHIUM SALT is often used in in vitro DNA synthesis reactions, as well as in techniques such as polymerase chain reaction (PCR) and DNA sequencing. The lithium salt form of 2'-deoxy-cytidine-5'-triphosphate helps stabilize the compound and improve its solubility, making it easier to work with in laboratory settings.

Upstream product

• 1032-65-1 cytidine 5'-(dihydrogen phosphate) 
• 800-73-7 dCDP 
• 951-77-9 2'-Deoxycytidine 
• 1233921-79-3 C₉H₁₂Cl₂N₃O₅P 

Downstream Products

• 800-73-7 dCDP 
• 110537-14-9 N4-(6-aminohexyl)-dCTP 
• 31747-59-8 5-iodo-2′-deoxycytidine triphosphate 
• 951-77-9 2'-Deoxycytidine 

Relevant articles and documents

P(V) Reagents for the Scalable Synthesis of Natural and Modified Nucleoside Triphosphates

Natural and modified nucleoside triphosphates impact nearly every major aspect of healthcare research from DNA sequencing to drug discovery. However, a scalable synthetic route to these molecules has long been hindered by the need for purification by high performance liquid chromatography (HPLC). Here, we describe a fundamentally different approach that uses a novel P(V) pyrene pyrophosphate reagent to generate derivatives that are purified by silica gel chromatography and converted to the desired compounds on scales vastly exceeding those achievable by HPLC. The power of this approach is demonstrated through the synthesis of a broad range of natural and unnatural nucleoside triphosphates (dNTPs and xNTPs) using protocols that are efficient, inexpensive, and operationally straightforward.

Synthetic method of nucleoside tetraphosphate

The invention discloses a synthetic method of nucleoside tetraphosphate. The synthetic method comprises the steps of carrying out selective phosphorylation reaction by virtue of nucleoside and a cyclic phosphorylation reagent, and carrying out oxidation and hydrolysis loop opening, so as to obtain nucleoside tetraphosphate. The structure of the cyclic phosphorylation reagent is represented by a formula I (shown in the description). According to the synthetic method, 5'-nucleoside tetraphosphate is selectively generated from nucleoside under the effect of the high-selectivity phosphorylation reagent, and 3'-OH (and 2'-OH) does not need to be protected in the process, namely that the generaiton of 3'(and 2'-)tetraphosphate can be effectively inhibited. Nucleoside tetraphosphate synthesized by virtue of the method has wide use ranges in the biology fields of DNA sequencing, labeling, extension and the like; currently, the selling prices is expensive, a synthetic method is complex, the reaction selectivity is poor; and the synthetic method provided by the invention is good in selectivity and easy in separation and purification, required experimental conditions are simple, and the synthetic processes are all conventional chemical reactions, so that the synthetic method is applicable to large-scale popularization and use.

Substrate specificity of T5 bacteriophage deoxyribonucleoside monophosphate kinase and its application for the synthesis of [α-32P]d/rNTP

Bacteriophage T5 deoxynucleoside monophosphate kinase (dNMP kinase, EC 2.7.4.13) is shown to catalyze the phosphorylation of both d2CMP and ribonucleotides AMP, GMP, and CMP, but does not phosphorylate UMP. For natural acceptors of the phosphoryl group, k m and k cat were found. The applicability of T5 dNMP kinase as a universal enzyme capable of the phosphorylation of labelled r/dNMP was shown for the synthesis of [α- 32P]rNTP and [α-32P]dNTP.