210344-95-9

Basic information

• Product Name: Z-DEVD-FMK
• Synonyms: Z-ASP-GLU-VAL-ASP-FLUOROMETHYLKETONE;Z-ASP(OME)-GLU(OME)-VAL-ASP(OME)-CH2F;Z-ASP(OME)-GLU(OME)-VAL-ASP(OME)-FLUOROMETHYLKETONE;Z-ASP(OME)-GLU(OME)-VAL-ASP(OME)-FMK;Z-ASP(OME)-GLU(OME)-VAL-DL-ASP(OME)-FLUOROMETHYLKETONE;Z-DEVD-FLUOROMETHYLKETONE;Z-DEVD-FMK;Z-D(OME)E(OME)VD(OME) FLUOROMETHYLKETONE
• CAS NO: 210344-95-9
• Molecular Formula: C30H41FN4O13
• Molecular Weight: 684.66
• Product Categories: Caspases/Apoptosis;peptide;Caspase/Related Products;Pepetides;Apoptosis;peptides;API;Inhibitors
• Mol File: 210344-95-9.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 914.2±65.0 °C(Predicted)
• Appearance: /
• Density: 1.260±0.06 g/cm3(Predicted)
• Storage Temp.: −20°C
• Solubility: DMSO/DMF: ≥20 mM
• PKA: 10.26±0.46(Predicted)
• CAS DataBase Reference: Z-DEVD-FMK(CAS DataBase Reference)
• NIST Chemistry Reference: Z-DEVD-FMK(210344-95-9)
• EPA Substance Registry System: Z-DEVD-FMK(210344-95-9)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 210344-95-9(Hazardous Substances Data)

Usage

Uses

Z-DEVD-FMK is an irrversible caspase-3 inhibitor used as an anaesthetic agent. In addition, it is proposed to increase the longevity of platelet storage.

General Description

A potent, cell-permeable, and irreversible inhibitor of caspase-3 as well as caspase-6, caspase-7, caspase-8, and caspase-10. When using with purified native or recombinant enzyme, pretreatment with an esterase is required. A 5 mM (250 μg/75 μl) solution of Z-DEVD-FMK (Cat. No. 264156) in DMSO is also available.

Biological Activity

Cell-permeable, irreversible inhibitor of caspase-3/CPP32; inhibits tumor cell apoptosis. Neuroprotective in rat hippocampus following seizures in vivo .

Biochem/physiol Actions

Cell permeable: yes

references

1. garcia-calvo m, peterson ep, leiting b, ruel r, nicholson dw, thornberry na (1998) inhibition of human caspases by peptidebased and macromolecular inhibitors. j biol chem 273:32608–32613 2. thornberry na, rano ta, peterson ep, rasper dm, timkey t, garcia-calvo m, houtzager vm, nordstrom pa, roy s, vaillancourt jp, chapman kt, nicholson dw (1997) a combinatorial approach defines specificities of members of the caspase family and granzyme b. functional relationships established for key mediators of apoptosis. j biol chem 272:17907–17911 3. yakovlev ag, knoblach sm, fan l, fox gb, goodnight r, faden ai (1997) activation of cpp32-like caspases contributes to neuronal apoptosis and neurological dysfunction after traumatic brain injury. j neurosci 17:7415–7424 4. clark rs, kochanek pm, watkins sc, chen m, dixon ce, seidberg na, melick j, loeffert je, nathaniel pd, jin kl, graham sh (2000) caspase-3 mediated neuronal death after traumatic brain injury in rats. j neurochem 74:740–753 5. s. m. knoblach, d. a. alroy et al, caspase inhibitor z-devd-fmk attenuates calpain and necrotic cell death in vitro and after traumatic brain injury, journal of cerebral blood flow & metabolism 24:1119–1132.