21150-22-1 Usage
Description
BETA-AMANITIN is a bicyclic octapeptide belonging to the family of amatoxins, which are naturally occurring toxins found in certain species of mushrooms, particularly Amanita phalloides. It is characterized by its acidic nature due to the presence of a carboxyl group. BETA-AMANITIN has been the subject of various scientific studies and applications, primarily due to its potent biological activity.
Uses
Used in Analytical Chemistry:
BETA-AMANITIN is used as a calibration standard for the quantification of the toxin using liquid chromatography-high resolution-mass spectrometry/mass spectrometry (LC-HR-MS/MS) methods. This application is crucial for accurate measurement and detection of BETA-AMANITIN in various samples.
BETA-AMANITIN is also utilized in determining its concentration in urine samples by capillary zone electrophoresis (CZE). This method aids in the diagnosis of mushroom poisoning and monitoring the effectiveness of treatment.
Furthermore, BETA-AMANITIN is employed in the analysis of toxic mushrooms by liquid chromatography coupled to time-of-flight mass spectrometry. This technique helps identify the presence of amatoxins in mushrooms, which is essential for public health and safety.
Used in Molecular Biology:
As a tool in molecular biology, BETA-AMANITIN plays a significant role in research and experimentation. It is known to inhibit RNA polymerase II, which is a crucial enzyme in the process of transcription. By blocking this enzyme, BETA-AMANITIN can be used to study the regulation of gene expression and the mechanisms underlying various cellular processes.
Acute toxicity
peritoneal-mouse LD50: 0.4 mg/kg; unnamed-mouse LD50: 0.4 mg/kg
Mechanism of action
β-Amanitin is a cyclic peptide found in several species of the Amanita genus, as well as other poisonous fungi. It inhibits RNA polymerase II and III but not RNA polymerase I or bacterial RNA polymerase, thus preventing mammalian protein synthesis. The biological activity of this toxin is similar to that of α-amanitin but differs structurally by the presence of a carboxyl group, which is useful for coupling reactions.
Biochem/physiol Actions
Toxic constituent of the mushroom, Amanita phalloides, inhibits eukaryotic RNA polymerase II and III, but not RNA polymerase I or bacterial RNA polymerase. Inhibits mammalian protein synthesis.
Check Digit Verification of cas no
The CAS Registry Mumber 21150-22-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,1,1,5 and 0 respectively; the second part has 2 digits, 2 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 21150-22:
(7*2)+(6*1)+(5*1)+(4*5)+(3*0)+(2*2)+(1*2)=51
51 % 10 = 1
So 21150-22-1 is a valid CAS Registry Number.
InChI:InChI=1/C39H53N9O15S/c1-4-16(2)31-36(60)41-11-28(53)42-25-15-64(63)38-21(20-6-5-18(50)7-22(20)45-38)9-23(33(57)40-12-29(54)46-31)43-37(61)32(17(3)27(52)14-49)47-35(59)26-8-19(51)13-48(26)39(62)24(10-30(55)56)44-34(25)58/h5-7,16-17,19,23-27,31-32,45,49-52H,4,8-15H2,1-3H3,(H,40,57)(H,41,60)(H,42,53)(H,43,61)(H,44,58)(H,46,54)(H,47,59)(H,55,56)/t16?,17?,19?,23-,24-,25-,26-,27-,31-,32-,64+/m0/s1
