2183-17-7 Usage
Description
ALPHA-NAPHTHYL PHOSPHATE DISODIUM SALT, also known as 1-Naphthyl phosphate disodium salt, is a chemical compound commonly utilized in various laboratory applications and techniques. It is known for its ability to interact with enzymes and proteins, making it a valuable tool in the field of biochemistry and molecular biology.
Uses
Used in Biochemical Research:
ALPHA-NAPHTHYL PHOSPHATE DISODIUM SALT is used as a substrate for determining acid phosphatase activity. It serves as a colorimetric indicator, allowing researchers to measure the activity of this enzyme in various samples.
Used in Gel Electrophoresis:
In the staining of alkaline phosphatase isoforms, ALPHA-NAPHTHYL PHOSPHATE DISODIUM SALT is used as a component in 7.5% non-denaturing polyacrylamide gels. This application aids in the visualization and differentiation of various isoforms of alkaline phosphatase, which are essential for understanding their functions and roles in biological processes.
Used in Molecular Imprinting:
ALPHA-NAPHTHYL PHOSPHATE DISODIUM SALT is used as a component in the mixture for preparing acid phosphomonoesterase-reactive imprinting sheets. This technique is employed to create molecularly imprinted polymers, which are highly selective and can be used for various analytical and separation purposes.
Used in Plant Biology:
As a component in the staining solution for staining roots, ALPHA-NAPHTHYL PHOSPHATE DISODIUM SALT is used for determining phosphomonoesterase activity in plants. This application helps researchers understand the role of these enzymes in plant growth, development, and response to environmental stimuli.
Purification Methods
Purify the salt through an acid ion-exchange column (in H+ form) to give the free acid [1136-89-6], M 224.2, which is obtained by freeze drying and recrystallising from Me2CO/*C6H6, or by adding 2.5volumes of hot CHCl3 (or 20 parts of boiling *C6H6) to a hot solution of 1 part acid and 1.2 parts Me2CO and cooling (m 155-157o, 157-158o). The acid is dissolved in the minimum volume of H2O to which 2 equivalents of NaOH are added and then freeze dried, or by adding the equivalent amount of MeONa in MeOH to a solution of the acid in MeOH and collecting the Na salt, washing with cold MeOH, then Et2O, and drying in a vacuum. [Friedman & Seligman J Am Chem Soc 72 624 1950, Chanley & Feageson J Am Chem Soc 77 4002 1955.] The monosodium salt [1136-89-6] is similarly prepared but by using 1 equivalent of NaOH. The phosphate group is hydrolysed at pH 1.1-5.85 at 70o. [Beilstein 6 IV 4226.] It is a substrate for alkaline phosphatase [Gomori Methods Enzymol 4 381 1957, 128 212 1968], and prostatic phosphatase [Babson Clin Chem 30 1418 1984]. [See p 538 in “Metal-organic Compounds”, Chapter 5, Beilstein 6 IV 4226.]
Check Digit Verification of cas no
The CAS Registry Mumber 2183-17-7 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 2,1,8 and 3 respectively; the second part has 2 digits, 1 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 2183-17:
(6*2)+(5*1)+(4*8)+(3*3)+(2*1)+(1*7)=67
67 % 10 = 7
So 2183-17-7 is a valid CAS Registry Number.
InChI:InChI=1/C10H9O4P.2Na/c11-15(12,13)14-10-7-3-5-8-4-1-2-6-9(8)10;;/h1-7H,(H2,11,12,13);;/q;2*+1/p-2
