2381-85-3

Basic information

• Product Name: NILE BLUE CHLORIDE
• Synonyms: NILE BLUE CHLORIDE;NILE BLUE HYDROCHLORIDE;NILE BLUE A CHLORIDE;5-amino-9-(diethylamino)-benzo(a)phenazoxoniuchloride;5-amino-9-(diethylamino)-benzo(a)phenoxazin-7-iuchloride;5-amino-9-(diethylamino)-benzo[a]phenoxazin-7-iuchloride;Nile Blue chloride, pure;Nile blue chloride, laser grade
• CAS NO: 2381-85-3
• Molecular Formula: C₂₀H₂₀N₃O*Cl
• Molecular Weight: 353.85
• EINECS: 219-181-4
• Product Categories: Organics;marker;Miscellaneous;Oxazine
• Mol File: 2381-85-3.mol
• Article Data: 2

Chemical Properties

• Melting Point: >250°C
• Boiling Point: 487.9°Cat760mmHg
• Flash Point: 248.8°C
• Appearance: Dark blue/Powder
• Density: g/cm³
• Solubility: Solubility Soluble in water, ethanol
• PKA: 9.7(at 25℃)
• Stability: Stable. Incompatible with strong oxidizing agents.
• BRN: 3923952
• CAS DataBase Reference: NILE BLUE CHLORIDE(CAS DataBase Reference)
• NIST Chemistry Reference: NILE BLUE CHLORIDE(2381-85-3)
• EPA Substance Registry System: NILE BLUE CHLORIDE(2381-85-3)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• RTECS: DJ1930000
• Hazardous Substances Data: 2381-85-3(Hazardous Substances Data)

Usage

Chemical Properties

crystalline solid

Uses

Nile Blue chloride is a histological and lipid stain.

Definition

ChEBI: An organic chloride salt having 5-amino-9-(diethylamino)benzo[a]phenoxazin-7-ium as the couterion. fluorescent dye which is also a potent photosensitiser for photodynamic therapy.

Preparation

commonly known as the Nile Blue. 5-(Diethylamino)-2-nitrosophenol?and Naphthalen-1-amine hydrochloride reaction.

Properties and Applications

bright blue. Soluble in hot water and ethanol are blue. The strong sulfuric acid for orange or brown, after dilute for blu-ray to green and then into a blue. The dye solution to join sodium hydroxide have red precipitation (precipitation soluble in ethyl ether for a green fluorescent light brown orange). Standard( Cotton ) Light Fastness Persperation Fastness Ironing Fastness Soaping Fading Stain Fading Stain Fading Stain A 1 2-3 3 1

Standard( Cotton )

Light Fastness

Fading

Stain

InChI:InChI=1/C20H20N3O.ClH/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;/h5-12H,3-4,21H2,1-2H3;1H/q+1;/p-1

Upstream product

• 93484-98-1 2-Hydroxy-4-imino-4H-naphthalen-1-one 
• 25912-14-5 2-amino-5-(diethylamino)phenol dihydrochloride 
• 23939-67-5 [1,4]naphthoquinone-bis-chlorimin 
• 91-68-9 3-diethylaminophenol 
• 612-52-2 2-naphthylamine hydrochloride 

Downstream Products

• 4128-37-4 1,3-diisopropylurea 

Relevant articles and documents

Transition-Metal-Mediated versus Tetrazine-Triggered Bioorthogonal Release Reactions: Direct Comparison and Combinations Thereof

Bioorthogonal cleavage reactions are gaining popularity in chemically inducible prodrug activation and in the control of biomolecular functions. Despite similar applications, these reactions were developed and optimized on different substrates and under different experimental conditions. Reported herein is a side-by-side comparison of palladium-, ruthenium- and tetrazine-triggered release reactions, which aims at comparing the reaction kinetics, efficiency and overall advantages and limitations of the methods. In addition, we disclose the possibility of mutual combination of the cleavage reactions. Finally, we compare the efficiency of the bioorthogonal deprotections in cellular experiments, which revealed that among the three methods investigated, the palladium- and the tetrazine-promoted reaction can be used for efficient prodrug activation, but only the tetrazine-triggered reactions proceed efficiently inside cells.