277760-34-6Relevant articles and documents
RNA polymerase transcription promoters and nucleic acid sequencing method
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, (2008/06/13)
An RNA polymerase transcription accelerator comprising a compound represented by the following Formula (I) or salts thereof. A method of sequencing DNA in which nucleic acid transcripts are obtained using an RNA polymerase and a DNA fragment as a template, the resulted nucleic acid transcripts are separated, the nucleic acid sequence is determined from the separated fractions wherein the nucleic acid transcription reaction is carried out in the presence of a compound selected from a group of compounds represented by the above formula (I). The polyamine compounds above have outstanding accelerating activity on transcription activity of RNA polymerase. Therefore, use of the polyamine compounds in a DNA sequencing method using RNA polymerase can make a length of DNA sequence that can be determined in one sequencing longer.
T7 RNA polymerase activation and improvement of the transcriptional sequencing by polyamines
Iwata, Masaaki,Izawa, Masaki,Sasaki, Nobuya,Nagumo, Yoko,Sasabe, Hiroyuki,Hayashizaki, Yoshihide
, p. 2185 - 2194 (2007/10/03)
We examined the possibility to improve the effectiveness of the in vitro transcription system using T7 RNA polymerase by coexistence with organic bases. The effect of the additives was evaluated by measuring the amount of RNA products in comparison with t
