28718-90-3 Usage
Description
DAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-T rich sequences of DNA. DAPI is often used as a counterstain, as its ultraviolet (max 358 nm) excitation and blue (max 461 nm) emission wavelengths separate it nicely from many popular primary fluorophores. It can be used on either fixed or live cells, although its low permeability in live cells demands that higher concentrations be used.
Chemical Properties
Yellow Solid
Uses
Different sources of media describe the Uses of 28718-90-3 differently. You can refer to the following data:
1. 4',6-Diamidino-2-phenylindole dihydrochloride is used as a cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine.It is used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes.
2. DAPI is used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex and as a cell permeable fluorescent minor groove-binding probe for DNA. For staining DNA in agarose gels
3. Suitable forDNA staining in agarose gelsanalysis of changes in DNA during apoptosisdetection of mycoplasmaphotofootprinting of DNAimmunofluorescent staining of cellsDAPI has been used:-in rapid monitoring of microbial contamination in chromosomal banding techniquein detection of apoptotic cellsin fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNAto stain mature pollen grains(0.5 mg/ml)
General Description
DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
References
Russell et al. (1975), A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses; Nature, 253 461
Tarnowski et al. (1991), DAPI as a useful stain for nuclear quantitation; Biotech Histochem., 66 297
Estandarte et al. (2016), The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes; Sci. Rep., 6 31417
Check Digit Verification of cas no
The CAS Registry Mumber 28718-90-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,8,7,1 and 8 respectively; the second part has 2 digits, 9 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 28718-90:
(7*2)+(6*8)+(5*7)+(4*1)+(3*8)+(2*9)+(1*0)=143
143 % 10 = 3
So 28718-90-3 is a valid CAS Registry Number.
28718-90-3Relevant articles and documents
Preparation method of 4', 6-diamidinyl-2-phenylindole dihydrochloride
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, (2021/06/26)
The invention discloses a preparation method of 4', 6-diamidinyl-2-phenylindole dihydrochloride, which comprises the following steps: firstly, taking 6-substituted indole as a raw material, and reacting with formamide under the action of a reaction solvent and a first catalyst to prepare 6-cyano indole; then performing a reactionthe 6-cyanoindole and the 4-substituted cyanobenzene under the action of a second catalyst, and obtaining 6-cyano-2-(4 '-cyanophenyl) indole; using 6-cyano-2-(4'-cyanophenyl) indole as a raw material and reacting with alcohol containing a substituent group under the action of a third catalyst, and obtaining 4', 6-bis (substituent group-containing methyleneimic acid ester)-2-phenylindole dihydrochloride; finally, continuously reacting with ammonium chloride, and obtaining the 4', 6-diamidinyl-2-phenylindole dihydrochloride. The design route is reasonable in design, the process safety is high, the method is friendly to operators, the cost is low, and the requirement for industrial production of products can be fully met.
