333338-18-4 Usage
Chemical Properties
White to off-white powder
Uses
Different sources of media describe the Uses of 333338-18-4 differently. You can refer to the following data:
1. Substrate for alkaline and acid phosphatase determinations4-Nitrophenyl phosphate disodium salt hexahydrate is a chromogenic substrate used in the determination of acid and alkaline phosphatases.
2. 4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISAalkaline phosphatase assay
3. Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.
General Description
Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
Biochem/physiol Actions
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.
Check Digit Verification of cas no
The CAS Registry Mumber 333338-18-4 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 3,3,3,3,3 and 8 respectively; the second part has 2 digits, 1 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 333338-18:
(8*3)+(7*3)+(6*3)+(5*3)+(4*3)+(3*8)+(2*1)+(1*8)=124
124 % 10 = 4
So 333338-18-4 is a valid CAS Registry Number.
InChI:InChI=1/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
