34994-50-8 Usage
Chemical Properties
Dark Purple Solid
Uses
Different sources of media describe the Uses of 34994-50-8 differently. You can refer to the following data:
1. Resorufin is a pink fluorescent dye that is not fluorescent until activated by UV light. Resorufin is an anionic tricyclic dye.
2. Resorufin has been used to determine the cytochrome P450 (CYP) 2A5 coumarin 7-hydroxylase (COH) activity changes over a broad range of lipopolysaccharide (LPS) doses. It has also been used as an internal standard for ethoxyresurufin-o-deethylase (EROD) analysis.
General Description
Resorufin is a fluorescent indicator.
Purification Methods
The free acid is purified by dissolving it in conc H2SO4 which on dilution precipitates resorufin and is filtered off, washed well with hot H2O and dried. [Eichler J Prakt Chem 139 113 1934.] Detailed UV and IR studies were reported by Musso & Mattheis Chem Ber 90 1814 1957]. [pKa: Musso & Rathjen Chem Ber 92 751 1959, Beilstein 27 1V 2263.]
The monoacetate [1152-14-3] M 252.2, m 2 2 3o is a fluorogenic substrate used for hydrolytic enzymes, and its fluorescence has Emax 593nm (Eex 500nm, in 0.1M phosphate pH 8.0 with lipase) [Kitson & Kitson Biochem J 322 701 1997]. [Beilstein 27 IV 2263.]
The O7-methylresorufin (7-methoxy-3H-phenoxazin-3-one) [5735-89-3] M 227.2, m > 2 2 0o has fluorescence at Emax 585nm (Eexcit 571 nm in deacylase solution) and is used to differentiate isoenzymes of cytochrome P-450. It is insoluble in H2O and dilute alkali but is soluble in EtOH and CHCl3 to give an orange yellow colour. [Kehrmann Justus Liebigs Ann Chem 372 352 1910, Beilstein 27 II 108.]
Check Digit Verification of cas no
The CAS Registry Mumber 34994-50-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,4,9,9 and 4 respectively; the second part has 2 digits, 5 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 34994-50:
(7*3)+(6*4)+(5*9)+(4*9)+(3*4)+(2*5)+(1*0)=148
148 % 10 = 8
So 34994-50-8 is a valid CAS Registry Number.
InChI:InChI=1/C12H7NO3.Na/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9;/h1-6,14H;/q;+1/p-1
34994-50-8Relevant articles and documents
A highly sensitive and selective fluorescence off-on probe for the detection of intracellular endogenous tyrosinase activity
Wu, Xiaofeng,Li, Xiaohua,Li, Hongyu,Shi, Wen,Ma, Huimin
, p. 2443 - 2446 (2017)
A resorufin-based highly sensitive and selective fluorescence off-on probe with a new recognition moiety for tyrosinase is developed, and applied to detect and image endogenous tyrosinase activity in different living cells.
Colorimetric and fluorescence analysis of percarbonate and oxygen bleach by perhydrolysis of resorufin ester
Ahn, Sangdoo,Chang, Suk-Kyu,Choi, Myung Gil,Kim, Na Young,Lee, Kang Min,Park, Ka Young,Yoo, Jae Hoon
, (2020/09/11)
Percarbonate is an important oxidant that is widely used in industrial applications and daily life. In this study, a simple colorimetric and fluorescence method for percarbonate determination using resorufin-based probe 1, carrying a pivaloyl protection group as a signaling switch, was developed. Probe 1 exhibited selective and sensitive signaling behavior for percarbonate via perhydrolysis of the pivaloyl group by hydrogen peroxide that was produced from percarbonate, in the presence of commonly encountered metal ions and anions. Among the esters tested as signaling switches, pivalate was optimal owing to its resistance to spontaneous hydrolysis and high signaling speed under the employed conditions. The detection limit for percarbonate was estimated to be 0.45 μM. The probe was applied to the analysis of percarbonate in domestic bleach using a scanner as a field-oriented signal-capturing device.
The catalysed NADH reduction of resazurin to resorufin
Candeias, Luis P.,MacFarlane, Donald P. S.,McWhinnie, Sean L. W.,Maidwell, Nicola L.,Roeschlaub, Carl A.,Sammes, Peter G.,Whittlesey, Rachel
, p. 2333 - 2334 (2007/10/03)
Details are reported on the mechanism whereby NADH can be used for the reduction of resazurin 1 to give the fluorescent product resorufin 2, a process requiring the use of a catalyst, such as N-methylphenazinium methosulfate 3.
