3520-42-1 Usage
Description
Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye that is extensively used for in vitro cytotoxicity screening. It binds to the protein constituents of cells in a stoichiometric manner, making the amount of dye extracted from stained cells directly proportional to the cell mass. SRB is also used for cell density determination and has various applications in different industries.
Uses
Used in Nonpermanent Tattoos:
SULFORHODAMINE B is used as a dye for creating nonpermanent tattoos.
Used in Acute Respiratory Distress Syndrome Treatment:
SULFORHODAMINE B is used as a potential new therapeutic agent for the treatment of acute respiratory distress syndrome.
Used in Environmental Testing:
SULFORHODAMINE B is used for relative total cell mass quantification, investigating the viscosity of coacervates, and as a molecular rotor for microviscosity determination in aerosol droplets.
Used in Cytotoxicity and Proliferation Assays:
SULFORHODAMINE B is used as a staining agent in the SRB assay for the determination of in vitro cytotoxicity, observation of live cells, proliferation, and drug screening.
Used in Blood-Brain Barrier Integrity Assessment:
SULFORHODAMINE B is used as a microscopic tracer for the assessment of blood-brain barrier integrity by post-mortem inspection of frozen sections.
Used in Dental Applications:
SULFORHODAMINE B is used as a caries detector dye with subsequent inspection of histological material.
Used in Textile Industry:
SULFORHODAMINE B is used as a dye for wool, silk, and polyamide fiber dyeing. It can also be used for wool and various fabric dyeing, as well as for direct printing on wool and silk fabrics, and for leather dyeing.
Standard:
The light fastness, soaping, perspiration fastness, oxygen bleaching, and fastness to seawater of SULFORHODAMINE B are rated according to ISO and AATCC standards, with ratings ranging from 1 to 4, indicating varying levels of resistance and performance.
Preparation
4-Formylbenzene-1,3-disulfonic acid and 3-(Diethylamino)phenol condensation, the products with sulfuric acid dehydration, and ferric chloride oxidation, and finally into sodium salt.
Flammability and Explosibility
Notclassified
Biochem/physiol Actions
Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening.
Standard
Light Fastness
Fading
Stain
ISO
3
AATCC
2-3
Check Digit Verification of cas no
The CAS Registry Mumber 3520-42-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,5,2 and 0 respectively; the second part has 2 digits, 4 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 3520-42:
(6*3)+(5*5)+(4*2)+(3*0)+(2*4)+(1*2)=61
61 % 10 = 1
So 3520-42-1 is a valid CAS Registry Number.
InChI:InChI=1/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
3520-42-1Relevant articles and documents
COMPOUND, RESIST COMPOSITION FOR COLOR FILTER, COLOR FILTER, DYED FIBER, AND DYEING METHOD
-
Paragraph 0089-0090; 0091, (2018/08/20)
An object is to provide a compound having high color developability and excellent heat resistance, and a resist composition for a color filter and a color filter that contain the dye composition. Another object is to provide a dyed polyamide fiber product having excellent wet rubbing fastness. The objects are achieved by a compound represented by the following general formula (1):
Activatable probes and methods for in vivo gene detection
-
, (2008/06/13)
Probes for detecting a target polynucleotide are provided. One aspect provides a molecular beacon probe set wherein the donor molecular beacon comprises a quantum dot and an acceptor molecular beacon comprises at least one reporter. The probes optionally comprise a protein transduction domain, targeting signal, or a combination thereof. Methods for detecting target polynucleotides using the disclosed probes are also provided.
Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
-
, (2008/06/13)
The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and/or acceptor moieties of molecular energy transfer pairs. The moieties can be fluorophores, such that fluorescent energy emitted by the donor is absorbed by the acceptor. The acceptor may be a fluorophore that fluoresces at a wavelength different from the donor moiety, or it may be a quencher. The oligonucleotides of the invention are configured so that a donor moiety and an acceptor moiety are incorporated into the amplification product. The invention also provides methods and kits for directly detecting amplification products employing the nucleic acid amplification primers. When labeled linear primers are used, treatment with exonuclease or by using specific temperature eliminates the need for separation of unincorporated primers. This "closed-tube" format greatly reduces the possibility of carryover contamination with amplification products, provides for high throughput of samples, and may be totally automated.