3654-96-4 Usage
Description
L-[35S]Methionine is a radioactive form of the essential amino acid methionine, labeled with the isotope sulfur-35. It is commonly used in molecular biology and biochemistry research to investigate protein synthesis, labeling specific proteins for in vivo and in vitro studies. By incorporating the radioactive sulfur into newly synthesized proteins, researchers can track protein turnover, study protein-protein interactions, and analyze protein localization within cells.
Used in Molecular Biology and Biochemistry Research:
L-[35S]Methionine is used as a radioactive tracer for investigating protein synthesis, allowing researchers to label specific proteins for in vivo and in vitro studies.
Used in Protein Turnover Studies:
L-[35S]Methionine is used as a tool to track protein turnover, providing insights into the rates at which proteins are synthesized and degraded within cells.
Used in Protein-Protein Interaction Analysis:
L-[35S]Methionine is used as a means to study protein-protein interactions, helping researchers understand how proteins interact and function within complex biological systems.
Used in Protein Localization Studies:
L-[35S]Methionine is used to analyze protein localization within cells, aiding in the identification of protein functions and their roles in cellular processes.
Used in Radiolabeling Assays:
L-[35S]Methionine is used in radiolabeling assays to detect protein synthesis and degradation, offering a method to measure the dynamics of protein production and breakdown.
Used in Autoradiography:
L-[35S]Methionine is used in autoradiography to visualize proteins on gels or membranes, providing a technique to study protein expression patterns and distribution.
Check Digit Verification of cas no
The CAS Registry Mumber 3654-96-4 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,6,5 and 4 respectively; the second part has 2 digits, 9 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 3654-96:
(6*3)+(5*6)+(4*5)+(3*4)+(2*9)+(1*6)=104
104 % 10 = 4
So 3654-96-4 is a valid CAS Registry Number.
3654-96-4Relevant articles and documents
Biotechnological production of high specific activity L-35S-cysteine and L-35S-methionine by using a diploid yeast Saccharomyces cerevisiae
Gajendiran,Jayachandran,Rao,Unny,Thyagarajan
, p. 571 - 576 (2007/10/02)
High specific activity L-35S-cysteine and L-35S-methionine were synthesised by using a wild type diploid strain of baker's yeast- Saccharomyces cerevisiae. Yeast cells were grown in a sulphur depleted synthetic medium in which Na235SO4 (50 mCi/ml) was supplemented as the sole sulphur source. The level of incorporation was 60% on an average. The protein hydrolysate of the cultured cells was subjected to paper and column chromatographic separations to get the individual L-35S-aminoacids. The radiochemical yields of cysteine and methionine were 6-7% and 18-20% respectively. The radiochemical purity of the products was >95%. The highest specific activity for the products obtained by employing this method was 1100 Ci/mmole from the starting material, Na235SO4, with a specific activity of 1350 Ci/mmole.
