40107-93-5Relevant articles and documents
RNA Cloaking by Reversible Acylation
Kadina, Anastasia,Kietrys, Anna M.,Kool, Eric T.
, p. 3059 - 3063 (2018)
We describe a selective and mild chemical approach for controlling RNA hybridization, folding, and enzyme interactions. Reaction of RNAs in aqueous buffer with an azide-substituted acylating agent (100–200 mm) yields several 2′-OH acylations per RNA strand in as little as 10 min. This poly-acylated (“cloaked”) RNA is strongly blocked from hybridization with complementary nucleic acids, from cleavage by RNA-processing enzymes, and from folding into active aptamer structures. Importantly, treatment with a water-soluble phosphine triggers a Staudinger reduction of the azide groups, resulting in spontaneous loss of acyl groups (“uncloaking”). This fully restores RNA folding and biochemical activity.
New artificial fluoro-cofactor of hydride transfer with novel fluorescence assay for redox biocatalysis
Zhang, Lei,Yuan, Jun,Xu, Yufang,Zhang, Y.-H. Percival,Qian, Xuhong
supporting information, p. 6471 - 6474 (2016/06/06)
A new artificial fluoro-cofactor was developed for the replacement of natural cofactors NAD(P), exhibiting a high hydride transfer ability. More importantly, we established a new and fast screening method for the evaluation of the properties of artificial cofactors based on the fluorescence assay and visible color change.
The Thermally-Controlled Chemoselective Reduction of 5H-Pyrrolopyridine-5,7(6H)-dione with Sodium Borohydride
Goto, Takehiko,Saito, Minoru,Sato, Ryu
, p. 4178 - 4180 (2007/10/02)
The title reaction produced either 2-(hydroxymethyl)nicotinamide or 6,7-dihydro-7-hydroxy-5H-pyrrolopyridin-5-one as the major product at room temperature or -20 deg C, respectively, along with the corresponding regioisomers as minor products.These novel compounds were converted to known compounds in order to establish their isomeric structures.