40615-36-9Relevant articles and documents
Process for the capture and reuse of the 4,4′-dimethoxytriphenylmethyl group during manufacturing of oligonucleotides
Guo, Zhiqiang,Pfundheller, Henrik M.,Sanghvi, Yogesh S.
, p. 415 - 417 (1998)
In a standard amidite coupling-based automated oligonucleotide synthesis campaign, the 4,4′-dimethoxytriphenylmethyl (DMT) protecting group is discarded as waste along with large amounts of dichloromethane. Herein, we report a straightforward and simple process for complete capture and recovery of the DMT group and dichloromethane, rendering the oligonucleotide manufacturing process safer and more economical.
Synthesis method of high-purity 4, 4 '-dimethoxytriphenylchloromethane
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Paragraph 0009; 0027; 0029-0030; 0032-0040; 0042-0043, (2021/03/13)
The invention provides a synthesis method of high-purity 4, 4 '-dimethoxytriphenylchloromethane, which comprises the following steps: by using anisole and trichlorotoluene as initial raw materials, carrying out F-C reaction, hydrochloric acid hydrolysis reaction and chlorination reaction to prepare a DMT-Cl crude product of which the purity is higher than 98.5%, and carrying out solvent crystallization to obtain a DMT-Cl finished product of which the purity is higher than 99.9%, thereby obtaining the DMT-Cl finished product yield higher than 98%. According to the method provided by the invention, on the basis of ensuring high reaction yield, the target product is prepared with high purity, impurity components contained in the target product are clear, the content of the impurity componentsis controlled, and particularly, hydroxyl impurities and acetylated impurities are controlled to be 0.05% or below.
Post-synthetic modification of nucleic acids by inverse Diels-Alder reaction
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, (2011/09/15)
The present invention concerns a method and a kit for the post-synthetic modification of nucleic acids via an inverse Diels-Alder reaction.