425399-05-9

Basic information

• Product Name: CASIN
• Synonyms: CASIN;2-(6-phenyl-2,3,4,9-tetrahydro-1H-carbazol-1-ylamino)ethanol
• CAS NO: 425399-05-9
• Molecular Formula: C20H22N2O
• Molecular Weight: 306.40148
• Mol File: 425399-05-9.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 577.8±50.0 °C(Predicted)
• Appearance: /
• Density: 1.23±0.1 g/cm3(Predicted)
• Storage Temp.: 2-8°C
• Solubility: ≥30.6 mg/mL in DMSO; insoluble in H2O; ≥19.5 mg/mL in EtOH
• PKA: 14.76±0.10(Predicted)
• CAS DataBase Reference: CASIN(CAS DataBase Reference)
• NIST Chemistry Reference: CASIN(425399-05-9)
• EPA Substance Registry System: CASIN(425399-05-9)

Safety Data

• Hazardous Substances Data: 425399-05-9(Hazardous Substances Data)

Usage

Description

CASIN, an indole-based small molecule, is a potent inhibitor of the cell division control protein 42 homolog (Cdc42), a member of the Rho family of GTPases. It plays a crucial role in regulating various signal transduction pathways, including filopodia function, cell polarity, and cell cycling. With an IC50 of 2 μM for inhibiting actin assembly in PIP2-stimulated actin polymerization assays, CASIN has been instrumental in studying the effects of Cdc42 on the division, survival, and differentiation of hematopoietic stem cells.

Uses

Used in Cell Biology Research:
CASIN is used as a Cdc42 inhibitor for studying its effects on the stimulation of extracellular vesicles (EVs) in intestinal epithelial cells. This application helps researchers understand the role of Cdc42 in cellular processes and its potential implications in various diseases.
Used in Mammalian Physiology Studies:
In mice jejunum studies, CASIN is used as a Cdc42 inhibitor to investigate the role of Cdc42 in the regulation of cellular processes and its potential impact on overall health and disease progression.
Used in Microbiology Research:
CASIN is used as a Cdc42 inhibitor for studying its effects on microbial adhesion-triggered endocytosis (MATE) vesicle morphology in segmented filamentous bacteria (SFB)-colonized mice. This application aids in understanding the complex interactions between host cells and microbial communities, which can have significant implications for host health and disease susceptibility.

Biochem/physiol Actions

CASIN is an inhibitor of Cdc42 GTPase, a small GTPase of the Rho-subfamily that plays important roles in cytoskeleton organization, cell cycle progression, signal transduction, and vesicle trafficking. CASIN generated a rejuvenated phenotype of Hematopoietic stem cells (HSCs), reversing the aging-related and polarity phenotype of aged HSCs to that of young HSCs in vivo..

in vitro

previous study found that treatment with casin at 5μm reduced the elevated level of active cdc42 observed in aged primitive hematopoietic cells to the level observed in young cells. however, casin treatment could not alter cell cycle status or apoptosis in aged lt-hscs. moreover, in response to the treatment of casin, lt-hscs from aged mice had a dose-dependent increase in the percentage of polarized cells, which is progressively indistinguishable from young cells. in addition, though casin acted on cdc42 activity transiently, the increase in the percentage of polar cells among aged lt-hscs that were induced by casin could remain stable for at least up to 6 hr after the withdrawal of casin [1].

in vivo

rat in-vivo study showed that casin could generated a rejuvenated phenotype of hematopoietic stem cells (hscs), reversing the aging-related and polarity phenotype of aged hscs to that of young hscs [1].

references

[1] florian mc,drr k,niebel a,daria d,schrezenmeier h,rojewski m,filippi md,hasenberg a,gunzer m,scharffetter-kochanek k,zheng y,geiger h. cdc42 activity regulates hematopoietic stem cell aging and rejuvenation. cell stem cell.2012 may 4;10(5):520-30.