53760-20-6 Usage
Description
Cytochalasin J is a cell-permeable fungal toxin derived from the fungus Aspergillus clavatus. It is known for its ability to disrupt the actin cytoskeleton in eukaryotic cells, making it a valuable tool in the study of actin polymerization and cytological research.
Uses
Used in Research and Development:
Cytochalasin J is used as a research tool for studying actin polymerization and the cytoskeleton in eukaryotic cells. Its ability to disrupt the actin cytoskeleton allows researchers to investigate the role of actin in various cellular processes and functions.
Used in Cytological Research:
Cytochalasin J is employed as a cytological research agent, particularly in the study of cell structure, function, and dynamics. By inhibiting actin polymerization, it helps researchers understand the importance of the actin cytoskeleton in cellular processes such as cell division, migration, and signal transduction.
Used in Pharmaceutical Industry:
Cytochalasin J is used as a potential therapeutic agent in the pharmaceutical industry. Its ability to disrupt the actin cytoskeleton makes it a candidate for the development of drugs targeting cancer cells, as the disruption of actin dynamics can lead to the inhibition of cell proliferation and migration.
Biological Activity
cytochalasin j can alter mitotic spindle microtubule organization and kinetochore structure.the cytochalasins are cell-permeable fungal metabolites inhibiting actin polymerization, which can alter diverse processes including cell movement, growth, phagocytosis, degranulation, and secretion.
in vitro
cytochalasin j was identified as a deacetylated analog of cytochalasin h that weakly inhibited actin assembly but significantly altered mitotic spindle microtubule organization and kinetochore structure [1]. in a previous study, cytochalasin j treatment of prometaphase cells reduced the number of kmts and the size and organization of the kinetochore lamina. moreover, in approximately 30% of cells treated with cytochalasin j, the failure of a small number of chromosomes to attach to spindle fibers could be documented. these chromosomes showed a significant change in the organization of the kinetochore laminae. light microscopic analyses of cells treated with cytochalasin j revealed loss of chromosome congression, with chromsomes usually located at the periphery of the spindle. cells treated with 10 microg/ml cytochalasin j for 10 min and released into tissue culture medium showed a resumption of chromosome motion within a few minutes, both during congression and anaphase [2].
references
[1] walling, e. a.,krafft, g.a. and ware, b.r. actin assembly activity of cytochalasins and cytochalasin analogs assayed using fluorescence photobleaching recovery. archives of biochemistry and biophysics 264(1), 321-332 (1988).[2] wrench, g. a. and snyder, j.a. cytochalasin j treatment significantly alters mitotic spindle microtubule organization and kinetochore structure in ptk1 cells. cell motility and the cytoskeleton 36(2), 112-124 (1997).
Check Digit Verification of cas no
The CAS Registry Mumber 53760-20-6 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,3,7,6 and 0 respectively; the second part has 2 digits, 2 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 53760-20:
(7*5)+(6*3)+(5*7)+(4*6)+(3*0)+(2*2)+(1*0)=116
116 % 10 = 6
So 53760-20-6 is a valid CAS Registry Number.
InChI:InChI=1/C28H37NO4/c1-17-9-8-12-21-25(31)19(3)18(2)24-22(15-20-10-6-5-7-11-20)29-26(32)28(21,24)23(30)13-14-27(4,33)16-17/h5-8,10-14,17-18,21-25,30-31,33H,3,9,15-16H2,1-2,4H3,(H,29,32)/b12-8+,14-13+/t17-,18+,21-,22-,23+,24-,25+,27-,28?/m0/s1
