59432-60-9Relevant articles and documents
Inulinase immobilisation in PAA/PEG composite for efficient fructooligosaccharides production
Dimitrovski, Darko,Krastanov, Albert,Temkov, Mishela,Velickova, Elena
, (2021/01/05)
Inulinase was immobilised by entrapment method in polyacrylamide/polyethylene glycol composite and evaluated for its efficiency for short-chain fructooligosaccharides (3–6 degrees of polymerisation) production in batch hydrolysis system. Aqueous two-phase
Enhancing fructooligosaccharides production by genetic improvement of the industrial fungus Aspergillus niger ATCC 20611
Zhang, Jing,Liu, Caixia,Xie, Yijia,Li, Ning,Ning, Zhanguo,Du, Na,Huang, Xirong,Zhong, Yaohua
, p. 25 - 33 (2017/04/06)
Aspergillus niger ATCC20611 is one of the most potent filamentous fungi used commercially for production of fructooligosaccharides (FOS), which are prospective components of functional food by stimulating probiotic bacteria in the human gut. However, current strategies for improving FOS yield still rely on production process development. The genetic engineering approach hasn't been applied in industrial strains to increase FOS production level. Here, an optimized polyethylene glycol (PEG)-mediated protoplast transformation system was established in A. niger ATCC 20611 and used for further strain improvement. The pyrithiamine resistance gene (ptrA) was selected as a dominant marker and protoplasts were prepared with high concentration (up to 108?g?1 wet weight mycelium) by using mixed cell wall-lysing enzymes. The transformation frequency with ptrA can reach 30–50 transformants per μg of DNA. In addition, the efficiency of co-transformation with the EGFP reporter gene (egfp) was high (approx. 82%). Furthermore, an activity-improved variant of β-fructofuranosidase, FopA(A178P), was successfully overexpressed in A. niger ATCC 20611 by using the transformation system. The transformant, CM6, exhibited a 58% increase in specific β-fructofuranosidase activity (up to 507?U/g), compared to the parental strain (320?U/g), and effectively reduced the time needed for completion of FOS synthesis. These results illustrate the feasibility of strain improvement through genetic engineering for further enhancement of FOS production level.
Screening of biocatalysts for transformation of sucrose to fructooligosaccharides
Ganaie, Mohd Anis,Gupta, Uma Shanker,Kango, Naveen
, p. 12 - 17 (2013/10/01)
Twenty microorganisms comprising of sixteen molds, two yeasts and two bacteria were evaluated for their ability to produce fructosyltransferase (FTase) and generate fructooligosaccharides (FOS) from sucrose. FTase production by these microorganisms was studied over a period of 120 h on medium containing 20% (w/v) sucrose as the sole carbon source. High FTase levels (35-31 U/ml) were observed in culture filtrates of Aspergillus flavus, Aspergillus niger, Aspergillus terreus and Penicillium islandicum. Higher concentrations of FOS were generated from 50% (w/v) sucrose using culture filtrates of A. flavus NFCCI 2364 (63.40%, w/w), A. niger SI 19 (54.94%, w/w), A. flavus NFCCI 2785 (44.61%, w/w), P. islandicum MTCC 4926 (43.56%, w/w), A. terreus NFCCI 2347 (24.17%, w/w) and Fusarium solani NFCCI 2315 (15.25%, w/w). Kestose, nystose and 1-fructofuranosyl nystose were the predominant oligosaccharides as revealed by HPLC analysis.