61970-00-1

Basic information

• Product Name: LUCIFERASE
• Synonyms: Luciferase,recominant;Beetle luciferase;Einecs 263-359-4;Firefly luciferin luciferase;Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing);Photinus pyralis luciferase;Luciferase recombinant, lyophil.;Luciola mingrelica luciferase
• CAS NO: 61970-00-1
• Molecular Weight: 0
• EINECS: 263-359-4
• Product Categories: Chemiluminescent compoundsAnalytical Enzymes;Purified EnzymesEnzyme Class Index;1.13.x.x Acting on single donors with incorporation of molecular oxygen;1.x.x.x Oxidoreductases;Biochemicals and Reagents;Luciferase;Luminescent Compounds/Detection;enzyme
• Mol File: 61970-00-1.mol
• Article Data: 0

Chemical Properties

• Flash Point: 111℃
• Appearance: white/powder
• Storage Temp.: −70°C
• CAS DataBase Reference: LUCIFERASE(CAS DataBase Reference)
• NIST Chemistry Reference: LUCIFERASE(61970-00-1)
• EPA Substance Registry System: LUCIFERASE(61970-00-1)

Safety Data

• Hazard Codes: Xi,Xn,T
• Statements: 36/37/38-22-52/53-45
• Safety Statements: 26-36/37-24/25-23-22-61-45-53
• WGK Germany: 3
• F: 3-10-21
• Hazardous Substances Data: 61970-00-1(Hazardous Substances Data)

Usage

Description

Luciferase, specifically firefly luciferase, is a light-generating enzyme that catalyzes an oxidative reaction using luciferin, ATP, and molecular oxygen to produce oxyluciferin, which yields visible light. It has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered a model to study protein-anesthetic interactions and is highly useful in cell biology and molecular biology as a reporter of gene function and for the quantification of ATP.

Uses

Used in Molecular and Cell Biology:
Luciferase is used as a reporter for genetic function and for the efficient detection and quantitation of ATP. This application is particularly important in studying various biological processes and understanding gene expression.
Used in High-Throughput Screening:
Luciferase is used as a component of lysis solution to measure luminescence signals, which is part of the study to determine chemical signals that can activate the karrikin insensitive 2 (KAI2) pathway. This application is crucial in high-throughput screening for drug discovery and other research purposes.
Used in Bioluminescence Research:
The bioluminescence of ATP-dependent luciferase is commonly used in reporter systems that are amenable to high-throughput screening. This application allows for the monitoring of various biological processes and interactions in a non-invasive and sensitive manner.
Used in Drug Discovery and Development:
Luciferase is employed in the development of novel drug delivery systems to enhance the applications and efficacy of certain compounds, such as gallotannin, against cancer cells. Various organic and metallic nanoparticles have been used as carriers for gallotannin delivery, aiming to improve its delivery, bioavailability, and therapeutic outcomes.

Biochem/physiol Actions

Firefly luciferase is a 62 kDa protein that catalyzes the production of light. The enzyme requires ATP, molecular oxygen, and the heterocyclic compound, firefly luciferin, to generate light in a two-step process. The light producing reaction is initiated by luciferin activation (adenylation of its carboxylate group) and proceeds in the presence of molecular oxygen to yield a photon of yellow-green light.