6578-06-9

Basic information

• Product Name: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
• Synonyms: 5-BROMO-4-CHLORO-3-INDOYL PHOSPHATE, P-TOLUIDINE SALT;5-BROMO-4-CHLORO-3-INDOXYL PHOSPHATE, P-TOLUIDINE SALT;5-BROMO-4-CHLORO-1H-INDOL-3-YL PHOSPHATE P-TOLUIDINE;5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE;5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE SALT;5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE 4-TOLUIDINE SALT;5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE, COMPOUNDED WITH 4-METHYLBENZENAMINE;5-BROMO-4-CHLORO-3'-INDOLYPHOSPHATE P-TOLUIDINE SALT
• CAS NO: 6578-06-9
• Molecular Formula: C₇H₉N*C₈H₆BrClNO₄P
• Molecular Weight: 433.63
• EINECS: 229-506-1
• Product Categories: Indoles;Simple Indoles;substrate
• Mol File: 6578-06-9.mol
• Article Data: 1

Chemical Properties

• Melting Point: 194-195℃
• Boiling Point: 676.2 °C at 760 mmHg
• Flash Point: 362.7 °C
• Appearance: /tablets
• Vapor Pressure: 2.65E-14mmHg at 25°C
• Storage Temp.: 2-8°C
• Solubility: DMF: 20 mg/mL
• Water Solubility: DMF: 20 mg/mL
• CAS DataBase Reference: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt(CAS DataBase Reference)
• NIST Chemistry Reference: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt(6578-06-9)
• EPA Substance Registry System: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt(6578-06-9)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38-40
• Safety Statements: 26
• RIDADR: 2811
• WGK Germany: 3
• F: 3-8-10
• HazardClass: 6.1
• PackingGroup: III
• Hazardous Substances Data: 6578-06-9(Hazardous Substances Data)

Usage

Chemical Properties

White to light brown powder

Uses

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

General Description

BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase?activity in solution.

InChI:InChI=1/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

Relevant articles and documents

Azeto triazolo pyrazine β-lactam antibacterial agents

A compound of formula (I) or a salt thereof: STR1 wherein R1 is an amino group, an azido group, or an acylamino group as found in antibacterially active penicillins or cephalosporins; R2 is a hydrogen atom or a C1-4 alkyl group; R3 is a hydrogen atom, or a C1-4 alkyl group optionally substituted by a carboxyl, carboxylic ester, hydroxy, C1-4 alkyloxy, acyloxy or heterocyclylthio group; and R4 is hydrogen or a readily removable carboxyl protecting group. The compounds wherein R1 is an acylamino group are found in known antibacterially active penicillins and cephalosporins and wherein R4 is a hydrogen, a pharmaceutically acceptable salting ion or an in vivo hydrolyzable ester forming radical may be formulated in pharmaceutical compositions. Processes for the preparation of the compound (I) are also described.