7240-37-1 Usage
Description
7-Aminoactinomycin D (7-AAD) is a fluorescent DNA dye that is commonly used for the detection or exclusion of non-viable cells in flow cytometric analysis, as it is generally excluded by live cells. It displays excitation spectra of 488, 546, and 578 nm and an emission spectrum of 650 nm. As 7-AAD is detected in the far red range of the spectrum, it exhibits minimal spectral overlap with commonly used probes, therefore it can be used in conjunction with probes such as FITC. 7-AAD has been used to evaluate apoptosis and cell-mediated cytotoxicity and to stain DNA in cells that have been fixed and permeabilized by a variety of methods.
Uses
Different sources of media describe the Uses of 7240-37-1 differently. You can refer to the following data:
1. Fluorescent DNA stain. A featured product for Apoptosis research.
2. 7-Aminoactinomycin D is a fluorescent DNA dye for apoptosis and flow cytomtery studies.
General Description
Free form: Excitation: 503 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 550 nmEmission: 675 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 672 nmDNA Complex: Excitation: 543 nm; 555 nmEmission: 655 nm; 665 nm
Biochem/physiol Actions
Primary Targetleukemias and sarcomas
References
1) Telieps?et al.?(2013)?Cellular-FLIP, Raji isoform (c-FLIP R) modulates cell death induction upon T-cell activation and infection, Eur. J. Immunol. 43 1499
2) Schmid?et al.?(2007)?Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D, Nat. Protoc.?2?187
3) Toba?et al.?(2000) Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia using 7AAD/PY, Eur.J.Haematol. 64 10
4) Lecoeur?et al.?(1997)?Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods, J. Immunol. Methods?209?111
5) Su?et al.?(1996)?Hematopoietic cell protein-tyrosine phosphatase-deficient motheaten mice exhibit T cell apoptosis defect, J. Immunol.?156?4198
Check Digit Verification of cas no
The CAS Registry Mumber 7240-37-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 7,2,4 and 0 respectively; the second part has 2 digits, 3 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 7240-37:
(6*7)+(5*2)+(4*4)+(3*0)+(2*3)+(1*7)=81
81 % 10 = 1
So 7240-37-1 is a valid CAS Registry Number.
InChI:InChI=1/C62H87N13O16/c1-26(2)42-59(85)74-21-17-19-36(74)57(83)70(13)24-38(76)72(15)48(28(5)6)61(87)89-32(11)44(55(81)66-42)68-53(79)34-23-35(63)30(9)51-46(34)65-47-40(41(64)50(78)31(10)52(47)91-51)54(80)69-45-33(12)90-62(88)49(29(7)8)73(16)39(77)25-71(14)58(84)37-20-18-22-75(37)60(86)43(27(3)4)67-56(45)82/h23,26-29,32-33,36-37,42-45,48-49H,17-22,24-25,63-64H2,1-16H3,(H,66,81)(H,67,82)(H,68,79)(H,69,80)
