75277-39-3

Basic information

• Product Name: HEPES sodium salt
• Synonyms: 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID SODIUM SALT;2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID SODIUM SALT;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID, DISODIUM SALT;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID HEMISODIUM SALT;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANE-SULFONIC ACID HALF-SODIUM SALT;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID SODIUM SALT BUFFER;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID SODIUM SALT;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID, SODIUM SALT
• CAS NO: 75277-39-3
• Molecular Formula: C₈H₁₇N₂O₄S*Na
• Molecular Weight: 260.29
• EINECS: 278-169-7
• Product Categories: Buffer;Biotechnology Performance Certified (BPC) BuffersBiological Buffers;Buffer SolutionsBiological Buffers;Good Buffers;Biological Buffers;Buffer Convenience Packaging;Buffers A to ZBiological Buffers
• Mol File: 75277-39-3.mol
• Article Data: 1

Chemical Properties

• Melting Point: 234 °C
• Appearance: White/dry powder
• Density: 1.504[at 20℃]
• Vapor Pressure: 0Pa at 25℃
• Storage Temp.: Store at RT.
• Solubility: H2O: 1 M at 20 °C, clear, colorless
• PKA: 7.5(at 25℃)
• Water Solubility: It is soluble in water.
• Sensitive: Hygroscopic
• Stability: Stable. Hygroscopic - protect from moisture. Incompatible with strong oxidizing agents.
• CAS DataBase Reference: HEPES sodium salt(CAS DataBase Reference)
• NIST Chemistry Reference: HEPES sodium salt(75277-39-3)
• EPA Substance Registry System: HEPES sodium salt(75277-39-3)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 3-10
• TSCA: Yes
• Hazardous Substances Data: 75277-39-3(Hazardous Substances Data)

Usage

Chemical Properties

white powder

Uses

Different sources of media describe the Uses of 75277-39-3 differently. You can refer to the following data:
1. HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.
2. HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

General Description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Flammability and Explosibility

Notclassified

Synthetic route

1-(2-hydroxyethyl)piperazine (103-76-4) + sodium 2-hydroxyethanesulfonate (1562-00-1) → 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (75277-39-3)

Conditions	Yield
sodium hydroxide at 204℃; under 4793.91 Torr; for 2.25h; Product distribution / selectivity; Autoclave;	94.6%
sodium hydroxide In water at 140 - 200℃; under 1587.51 - 5414.51 Torr; for 2 - 72h; Product distribution / selectivity; Autoclave;	6%

Upstream product

• 103-76-4 1-(2-hydroxyethyl)piperazine 
• 1562-00-1 sodium 2-hydroxyethanesulfonate 

Relevant articles and documents

Preparation of substituted alkanesulfonates from 2-hydroxyalkanesulfonates

The present invention relates to a process for the preparation of substituted alkanesulfonates by the reaction of an aqueous metal substituted 2-hydroxyethanesulfonate with a nucleophile. 2-Alkylaminoalkanesulfonate is formed when the nucleophile is an amine. The invention also relates to the optimization of reaction conditions to produce an optimum yield.