79838-05-4Relevant articles and documents
4-nitrophenyl 4-morpholinylphosphonochloridate: a convenient reagent for the synthesis of ribonucleoside mono-, di- and tri-phosphates
Hartog, J. A. J. den,Boom, J. H. van
, p. 285 - 290 (2007/10/02)
The synthesis of the crystalline and easily accessible phosphorylating agent 4-nitrophenyl 7-morpholinylphosphonochloridate is reported.Its application to the preparation of 5'-phosphorylated ribonucleosides (e.g. pA, ppA and pppA) as well as RNA fragments (e.g. pUpU and ppUpU) is demonstrated.Further, special attention is paid to the mild removal of the 4-nitrophenyl group from fully protected phosphotriester intermediates.