839730-93-7

Basic information

• Product Name: MCA-GLY-LYS-PRO-ILE-LEU-PHE-PHE-ARG-LEU-LYS(DNP)-D-ARG-NH2
• Synonyms: MCA-GLY-LYS-PRO-ILE-LEU-PHE-PHE-ARG-LEU-LYS(DNP)-D-ARG-NH2;7-METHOXYCOUMARIN-4-ACETYL-GLY-LYS-PRO-ILE-LEU-PHE-PHE-ARG-LEU-LYS(DNP)-D-ARG-AMIDE
• CAS NO: 839730-93-7
• Molecular Formula: C84H120N22O19
• Molecular Weight: 1741.99
• Product Categories: Cathepsin DCathepsin/Granzyme Inhibitors&Substrates;Cathepsin DEnzyme Substrates;Cathepsin E SubstrateLysosomal Proteases;Cathepsin ECell Signaling Enzymes;Apoptosis Enzymes;Cathepsin;Cathepsins and Granzymes;Substrates;Substrates by Enzyme;SubstratesMore...Close...
• Mol File: 839730-93-7.mol
• Article Data: 0

Chemical Properties

• Appearance: /solid
• Storage Temp.: −20°C
• CAS DataBase Reference: MCA-GLY-LYS-PRO-ILE-LEU-PHE-PHE-ARG-LEU-LYS(DNP)-D-ARG-NH2(CAS DataBase Reference)
• NIST Chemistry Reference: MCA-GLY-LYS-PRO-ILE-LEU-PHE-PHE-ARG-LEU-LYS(DNP)-D-ARG-NH2(839730-93-7)
• EPA Substance Registry System: MCA-GLY-LYS-PRO-ILE-LEU-PHE-PHE-ARG-LEU-LYS(DNP)-D-ARG-NH2(839730-93-7)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• Hazardous Substances Data: 839730-93-7(Hazardous Substances Data)

Usage

Description

MCA-GLY-LYS-PRO-ILE-LEU-PHE-PHE-ARG-LEU-LYS(DNP)-D-ARG-NH2, also known as 7-methoxycoumarin-4-acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys-(DNP)-DArg-amide, is a synthetic peptide substrate designed for the quantification of cathepsin D activity in decapod crustaceans. It consists of a series of amino acids, including glycine, lysine, proline, isoleucine, leucine, phenylalanine, arginine, and D-arginine, with a 7-methoxycoumarin-4-acetyl group attached to the N-terminus and a D-arginine amide at the C-terminus. This specific sequence allows for the detection and measurement of cathepsin D activity, a lysosomal protease enzyme involved in various biological processes.

Uses

Used in Biochemical Research:
MCA-GLY-LYS-PRO-ILE-LEU-PHE-PHE-ARG-LEU-LYS(DNP)-D-ARG-NH2 is used as a fluorogenic substrate for the quantification of cathepsin D activity in decapod crustaceans. The substrate is specifically cleaved by cathepsin D, releasing the 7-methoxycoumarin-4-acetyl group and generating a fluorescent signal. This allows researchers to measure the activity of cathepsin D in various samples, providing valuable insights into the enzyme's role in biological processes and potential involvement in diseases.
Used in Pharmaceutical Development:
MCA-GLY-LYS-PRO-ILE-LEU-PHE-PHE-ARG-LEU-LYS(DNP)-D-ARG-NH2 can be used in the development of drugs targeting cathepsin D or related enzymes. By understanding the substrate specificity and cleavage sites of cathepsin D, researchers can design inhibitors or modulators of the enzyme, which may have therapeutic applications in treating diseases associated with abnormal cathepsin D activity.
Used in Diagnostic Applications:
The substrate can be employed in diagnostic assays to detect and measure cathepsin D activity in clinical samples. This may help in the diagnosis and monitoring of diseases where cathepsin D activity is altered, such as certain types of cancer or lysosomal storage disorders.
Used in Environmental Monitoring:
As the substrate is specific to decapod crustaceans, it can be used in environmental monitoring studies to assess the health of these organisms and their ecosystems. Changes in cathepsin D activity may indicate exposure to environmental stressors or pollutants, providing valuable information for conservation and management efforts.