84522-15-6 Usage
General Description
Naphthol AS-OL phosphate is a chemical compound used as a colorimetric reagent for the determination of alkaline phosphatase activity in various biological samples. It is a colorless substrate that can be hydrolyzed by alkaline phosphatase to yield a water-insoluble, red dye called naphthol. This reaction can be measured spectrophotometrically at a specific wavelength to quantify the enzyme activity. Naphthol AS-OL phosphate is commonly used in clinical and biochemical laboratories to diagnose various health conditions, including liver and bone diseases, as well as to monitor the effectiveness of treatment. Additionally, it is also used as a staining reagent in histochemical techniques for the localization of phosphatase enzymes in tissues.
Check Digit Verification of cas no
The CAS Registry Mumber 84522-15-6 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,4,5,2 and 2 respectively; the second part has 2 digits, 1 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 84522-15:
(7*8)+(6*4)+(5*5)+(4*2)+(3*2)+(2*1)+(1*5)=126
126 % 10 = 6
So 84522-15-6 is a valid CAS Registry Number.
InChI:InChI=1/C18H16NO6P/c1-24-16-9-5-4-8-15(16)19-18(20)14-10-12-6-2-3-7-13(12)11-17(14)25-26(21,22)23/h2-11H,1H3,(H,19,20)(H2,21,22,23)
84522-15-6Relevant articles and documents
Fluorescent substrates for potential use in enzyme-linked immunosorbent assay of membrane-bound nucleic acids
Fujita, Satoshi,Momiyama, Masayoshi,Kondo, Yasumitsu,Kagiyama, Naoto,Hori, Samuel H.,Toru, Takeshi
, p. 1347 - 1353 (2007/10/02)
A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2′-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2′-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of λ DNA can be successfully detected on the membrane with HBNP.
