9001-96-1

Basic information

• Product Name: PYRUVATE OXIDASE
• Synonyms: EC 1.2.3.3;PYRUVATE OXIDASE;PYRUVATE OXIDASE, AEROCOCCUS VIRIDANS;PYRUVATE: OXYGEN OXIDOREDUCTASE [PHOSPHORYLATING];Oxidase, pyruvate;PYRUVATE OXIDASE FROM AEROCOCCUS SP.;PYRUVATE OXIDASE, BACTERIAL*MICROORGANIS M;PYRUVATE OXIDASE FROM: MICROORGANISM (EC 1.2.3.3)
• CAS NO: 9001-96-1
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 232-644-5
• Mol File: 9001-96-1.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: −20°C
• CAS DataBase Reference: PYRUVATE OXIDASE(CAS DataBase Reference)
• NIST Chemistry Reference: PYRUVATE OXIDASE(9001-96-1)
• EPA Substance Registry System: PYRUVATE OXIDASE(9001-96-1)

Safety Data

• Safety Statements: 24/25
• WGK Germany: 3
• Hazardous Substances Data: 9001-96-1(Hazardous Substances Data)

Usage

Description

Pyruvate Oxidase is an enzyme that catalyzes the oxidation of pyruvate to acetyl-CoA, CO2, and H2O2. It is derived from various sources, including Aerococcus sp. and other microorganisms. This enzyme plays a crucial role in the metabolic pathways and has been widely used in various applications due to its unique properties.

Uses

Used in Research and Diagnostic Applications:
Pyruvate Oxidase is used as a key component in the Amplex Red-based fluorescence assay for pyruvate. This assay is a sensitive and specific method for detecting and quantifying pyruvate levels in various samples, making it valuable for research and diagnostic purposes.
Used in Biosensor Development:
Pyruvate Oxidase is utilized in the preparation of biosensors for the determination of thiamine (vitamin B1). These biosensors offer a convenient and accurate way to measure thiamine levels, which is essential for various biological processes and maintaining overall health.
Used in Medical Diagnostics:
Pyruvate Oxidase is employed as a component in the constant part of the multi-enzyme biocatalytic cascade for the determination of biomarkers for traumatic brain injury (TBI) and soft tissue injury (STI). This application aids in the early diagnosis and monitoring of these injuries, enabling timely medical intervention.
Used in Near Real-Time Continuous Detection Systems:
Pyruvate Oxidase from microorganisms has been used in developing near real-time continuous detection systems for pyruvate. These systems provide rapid and reliable measurements of pyruvate levels, which can be crucial for various applications, including medical diagnostics and monitoring metabolic processes.
Chemical Properties:
Pyruvate Oxidase is available in the form of a powder, making it easy to store, handle, and incorporate into various applications. Its powder form also allows for precise control over the amount used in different assays and systems, ensuring accurate and consistent results.

Biochem/physiol Actions

Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.