9005-64-5

Basic information

• Product Name: Tween 20
• Synonyms: Polisorbate 20- PS 20;Tween 20 (Trademark of ICI America, Inc.);Tween 20 Tween 20 1LT;Twain 20;TWEEN(R) 20 Vetec(TM) reagent grade, 40%;ACRYL/BIS 37.5:1 PREMIXED PWD ULTRA PURE;PESTLE AND TUBE 1.5ML ASSORTED
• CAS NO: 9005-64-5
• Molecular Weight: 522.6692
• EINECS: 500-018-3
• Product Categories: detergent;#N/A;Ester Ether Type (Surfactants);Functional Materials;Nonionic Surfactants;Surfactants;Biochemistry;Nonionic Surfactants (for Biochemistry);Surfactants (for Biochemistry);Alphabetical Listings;Flavors and Fragrances;O-P
• Mol File: 9005-64-5.mol
• Article Data: 1

Chemical Properties

• Melting Point: 98.9 °C (decomp)
• Boiling Point: 100 °C
• Flash Point: >230 °F
• Appearance: Amber/viscous liquid
• Density: 1.11 g/mL at 20 °C
• Vapor Pressure: 2.16E-22mmHg at 25°C
• Refractive Index: n20/D 1.468(lit.)
• Storage Temp.: 0-6°C
• Solubility: 100 g/L
• Water Solubility: 100 g/L
• Stability: Stable. Incompatible with strong oxidizing agents.
• CAS DataBase Reference: Tween 20(CAS DataBase Reference)
• NIST Chemistry Reference: Tween 20(9005-64-5)
• EPA Substance Registry System: Tween 20(9005-64-5)

Safety Data

• Hazard Codes: Xi
• Statements: 22-36/37/38-37/38-41
• Safety Statements: 24/25-36/37/39-36-26
• WGK Germany: 1
• RTECS: TR7400000
• F: 10-23
• TSCA: Yes
• Hazardous Substances Data: 9005-64-5(Hazardous Substances Data)

Usage

Description

Tween 20, whose common commercial names includes Alkest TW 20 and Polysorbate 20, is a mild nonionic surfactant formed by the ethoxylation of sorbitan before the addition of lauric acid which is a medium-chain fatty acid found mainly in coconut oil. It is allowed to be used as the emulsifier, detergent, dispersant, solvent and stabilizer, etc. in a number of fields such as pharmaceutical, chemical, food, textile and other industries due to its stability and relative non-toxicity. Tween 20 is widely applied in biological techniques and sciences, which can be added to buffers and reagents for immunohistochemistry, such as Western blots and ELISAs, helping to prevent non-specific antibody binding, decrease background staining and enhance reagent spreading. Besides, Tween 20 also has applications in food production as a common food grade additive which are found in many consumables on the market today. It can also be used as a wetting agent in flavored mouth drops such as Ice Drops, in oral or non-gastrointestinal suspensions and in rubber balers in the elastomer industry.

References

https://en.wikipedia.org/wiki/Polysorbate_20 http://www.organic-creations.com/thickeners-stabilizers/599-polysorbate-20

Chemical Properties

Different sources of media describe the Chemical Properties of 9005-64-5 differently. You can refer to the following data:
1. Polysorbate 20 is a mixture of laurate partial esters of sorbitol and sorbitol anhydrides condensed with approximately 20 mole of ethylene oxide (C2H4O) for each mole of sorbitol and its mono- and dianhydrides. It has a faint, characteristic odor and a warm, somewhat bitter taste.
2. Yellow liquid

Uses

Different sources of media describe the Uses of 9005-64-5 differently. You can refer to the following data:
1. Used for solubilizing membrane proteins during isolation and purification.Polysorbate 20 is used as a wetting agent in flavored mouth drops. It acts as a washing agent in Western blots and ELISAs and prevents antibody binding. It is a solubilizing agent of membrane proteins. In pharmaceutical chemistry, it is used as an excipient to stabilize emulsions and suspensions. Further, it is used as a dispersing agent, stabilizer, lubricator and also used in creams, salves, ointments, protective creams, balms, pomades, lipsticks, mascaras and glosses.
2. polysorbate 20 is a solubilizer, emulsifier, viscosity modifier, and stabilizer of essential oils in water.

Preparation

polysorbate 20 is prepared by condensing the partial esters of sorbitol and its anhydrides with ethylene oxide to effect an oxyethylene copolymerization at the free hydroxyl groups.

Definition

ChEBI: Polysorbate 20 (Tween 20) is a polymer composed of PEG-ylated sorbitan, where the total number of poly(ethylene glycol) units is 20 (w + x + y + z = 20) and a single terminal is capped by a dodecanoyl group. It is a surfactant and emulsifier used in cleaners and personal care products.

Brand name

Tween 20 (ICI Americas).

General Description

Polysorbate 20 is a hydrophilic nonionic surfactant generally used as emulsifiers, dispersing agent and solubilizer.

Health effects

Polysorbate 20 was a skin irritant in dermatitis patients but was reported not to induce irritant responses when applied to the intact skin of health volunteers. It produced, at worst, minimal irritation in the eyes of rabbits. Only a small number of cases of skin sensitization have been reported in humans, although a moderate to strong sensitizing potential was seen in guinea-pigs treated by intradermal injection.

Flammability and Explosibility

Nonflammable

Safety Profile

Moderately toxic by intravenous route. Mddly toxic by ingestion. A human skin irritant. When heated to decomposition it emits acrid smoke and irritating fumes.

InChI:InChI=1/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

Relevant articles and documents

Method of preparing hepatitis B core antigen

HBc Ag is prepared by isolating Dane particles by isopycnic banding of fluid from HBs Ag positive donors, optionally but preferably pelleting the Dane particles, and then removing the surface antigen by contacting the Dane particles with a nonionic surfactant having from about 15 to about 35 oxyethylene units in the presence of a reducing agent such as mercaptoethanol. The isolated core antigen is used as a diagnostic and immunologic agent.