9012-63-9

Basic information

• Product Name: CHOLERA TOXIN
• Synonyms: CHOLERA TOXIN;CHOLERA ENTEROTOXIN;CHOLERA TOXIN, VIBRIO CHOLERAE, TYPE INABA 569B;VIBRIO CHOLERAE TOXIN;choleraentero-exotoxin;choleraexotoxin;choleragen;exo-enterotoxin
• CAS NO: 9012-63-9
• Molecular Formula: NULL
• Molecular Weight: 0
• Mol File: 9012-63-9.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• Storage Temp.: 0-6°C
• Solubility: H2O: soluble10mg/mL
• CAS DataBase Reference: CHOLERA TOXIN(CAS DataBase Reference)
• NIST Chemistry Reference: CHOLERA TOXIN(9012-63-9)
• EPA Substance Registry System: CHOLERA TOXIN(9012-63-9)

Safety Data

• Hazard Codes: T
• Statements: 24/25-52/53
• Safety Statements: 36/37-45-61
• RIDADR: 3172
• WGK Germany: 2
• RTECS: LF3100000
• HazardClass: 6.1
• PackingGroup: I
• Hazardous Substances Data: 9012-63-9(Hazardous Substances Data)

Usage

Uses

Cholera Toxin from Vibrio cholerae has been used as a positive control in cAMP (cyclic AMP) assay for enterotoxins. It has been added as a supplement in cell culture media of primary tumors and epithelial cells.

Biochem/physiol Actions

Toxin consisting of an A subunit (27 kDa) surrounded by five B subunits (approximately 12 kDa each), which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein (Gαs), reducing GTPase activity and activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.