9025-64-3

Basic information

• Product Name: DEOXYRIBONUCLEASE II
• Synonyms: DEOXYRIBONUCLEASE II;DEOXYRIBONUCLEASE II TYPE IV;DEOXYRIBONUCLEASE II TYPE V;DEOXYRIBONUCLEATE 3'-OLIGONUCLEOTIDEHYDROLASE;DEOXYRIBONUCLEATE 3'-OLIGONUCLEOTIDOHYDROLASE TYPE IV;DEOXYRIBONUCLEATE 3'-OLIGONUCLEOTIDOHYDROLASE TYPE V;DEOXYIBONUCLEASE 3'-OLIGO NUCLEOTIDOHYDROLASE;DEOXYRIBONUCLEASE 3'-OLIGO-NUCLEOTIDOHYDROLASE
• CAS NO: 9025-64-3
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 232-801-8
• Mol File: 9025-64-3.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: −20°C
• CAS DataBase Reference: DEOXYRIBONUCLEASE II(CAS DataBase Reference)
• NIST Chemistry Reference: DEOXYRIBONUCLEASE II(9025-64-3)
• EPA Substance Registry System: DEOXYRIBONUCLEASE II(9025-64-3)

Safety Data

• WGK Germany: 3
• F: 10-21
• TSCA: Yes
• Hazardous Substances Data: 9025-64-3(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 9025-64-3 differently. You can refer to the following data:
1. Deoxyribonuclease II from bovine spleen has been used: as a component of digestion mix to isolate immune cells from colon samplesto digest retinal explants for single-cell suspension preparationfor isolation of stromal cells from human colon tissuein the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart
2. Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

Biochem/physiol Actions

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.