9027-60-5

Basic information

• Product Name: EC 4.1.3.3
• Synonyms: EC 4.1.3.3;N-ACETYLNEURAMINIC ACID ALDOLASE;N-ACETYLNEURAMINIC ACID ALDORASE;N-ACETYLNEURAMINATE PYRUVATE LYASE;NEU5AC ALDOLASE;SIALIC ALDOLASE;SIALIC ACID ALDOLASE;N-Acetylneuraminic acid Aldolase Escherichia coli
• CAS NO: 9027-60-5
• Molecular Weight: 0
• Product Categories: Lyases;Specialty Enzymes
• Mol File: 9027-60-5.mol
• Article Data: 0

Chemical Properties

• Appearance: slightly yellow/powder
• Storage Temp.: −20°C
• BRN: 2697172
• CAS DataBase Reference: EC 4.1.3.3(CAS DataBase Reference)
• NIST Chemistry Reference: EC 4.1.3.3(9027-60-5)
• EPA Substance Registry System: EC 4.1.3.3(9027-60-5)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 23-24/25-60-37-26-22
• WGK Germany: 3
• F: 10
• Hazardous Substances Data: 9027-60-5(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 9027-60-5 differently. You can refer to the following data:
1. Sialic acid aldolase can be used to synthesize unnatural sugars of C(6) to C(10) for the design of antagonists and inhibitors of glycoenzymes.
2. This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid whencoupled with the related enzymes in clinical analysis.For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.

General Description

Sialic acid aldolases, or N-acetylneuraminate lyases, catalyze the reversible aldol cleavage of N-acetylneuraminic acid to form pyruvate and N-acetyl-D-mannosamine. In nature, N-acetylneuraminate lyase mainly occurs in pathogens.

Purification Methods

Purify the aldolase by extraction with H2O, protamine precipitation, (NH4)2SO4 fractionation, Me2CO precipitation, acid treatment at pH 5.7 and precipitation at pH 4.5. The equilibrium constant for pyruvate + n-acetyl-D-mannosamine N-acetylneuraminidate at 37o is 0.64. The Km for N-acetylneuraminic acid is 3.9mM in phosphate at pH 7.2 and 37o. [Comb & Roseman Methods Enzymol 5 391 1962.] The enzyme from hog kidney (cortex) has been purified 1700-fold by extraction with H2O, protamine sulfate precipitation, (NH4)2SO4 fractionation, heating between 60-80o, a second (NH4)2SO4 fractionation and starch gel electrophoresis. The Km for N-acetylneuraminic acid is 1.5mM. [Brunetti et al. J Biol Chem 237 2447 1962.]