9036-19-5 Usage
Uses
Different sources of media describe the Uses of 9036-19-5 differently. You can refer to the following data:
1. Triton? X-100 has been used:In immunohistochemistry for staining the Flat-mount retinasAlong with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assayTo permeabilise cells during Immunofluorescent microscopic studiesAs a positive control in LDH assay to determine the cell membrane integrityFor estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedureFor the preparation of outer membrane protein exctractAs a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cellsIn the treatment of tissue sections for Immunofluorescence labeling
2. 0·3% Triton? X-100 in TBS has been used for permeabilization of retinas before immunostaining. 0.5% Triton X-100 has been used for astrocyte cell lysis.
3. Triton?-X has been used:In the permeabilization of cells for immunofluorescence stainingAs a component of lysis buffer in western blot analysisAs a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope
General Description
Octophenolpoly(ethyleneglycolether)nEspecially used for purification in membrane researchTriton X-100 is a water soluble, non-ionic surfactant. It exhibits a strong hydrogen bonding with water molecule in temperature dependent manner. It has a wide application area such as in isolation and solubilization of protein, DNA extraction and emulsification.
Biochem/physiol Actions
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
Check Digit Verification of cas no
The CAS Registry Mumber 9036-19-5 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,3 and 6 respectively; the second part has 2 digits, 1 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 9036-19:
(6*9)+(5*0)+(4*3)+(3*6)+(2*1)+(1*9)=95
95 % 10 = 5
So 9036-19-5 is a valid CAS Registry Number.
InChI:InChI=1/C18H30O3/c1-3-5-6-7-8-12-15-18(19,21-4-2)16-20-17-13-10-9-11-14-17/h9-11,13-14,19H,3-8,12,15-16H2,1-2H3