9046-56-4

Basic information

• Product Name: ANCROD
• Synonyms: a38414(enzyme);abbott38414;ancrod;arvin;arwin;irc-50arvin;proteinase,agkistrodonrhodostomavenom;Proteinase, Agkistrodon serine
• CAS NO: 9046-56-4
• Molecular Weight: 0
• EINECS: 232-933-6
• Mol File: 9046-56-4.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• Storage Temp.: −20°C
• CAS DataBase Reference: ANCROD(CAS DataBase Reference)
• NIST Chemistry Reference: ANCROD(9046-56-4)
• EPA Substance Registry System: ANCROD(9046-56-4)

Safety Data

• Hazard Codes: Xn
• Statements: 36/37/38-42
• Safety Statements: 22-24-26-36/37
• RTECS: UK9575000
• Hazardous Substances Data: 9046-56-4(Hazardous Substances Data)

Usage

Originator

Viprinex,Knoll Pharmaceuticals

Manufacturing Process

Triethylaminoethyl cellulose powder (Serva) of capacity 0.71 m.equiv./gm is suspended in 2 M sodium chloride buffered with 0.1 M tris/phosphate pH 6.0 and the slurry is packed into a glass column 3.6 cm in diameter until the height of the packed material reaches 20 cm. The column is washed with a further 2 L of the solvent used for preparing the slurry and is then equilibrated with 0.01 M tris/phosphate buffer pH 8.5. Tris is an abbreviation for tris(hydroxymethyl)aminomethane. 330-360 mg of crude A. rhodostoma venom is dissolved in 20 ml of 0.01 M tris/phosphate buffer pH 8.5, centrifuged to remove insoluble material, and the clear supernatant is applied to the column. The fractionation is carried out at room temperature at a flow rate of 90-100 ml (35 ml/hour). The protein concentration in the eluate is estimated from the extinction of the solution at 280 m/t in 1 cm cells. The chromatogram is developed with the following buffers. In all cases the molarity of the buffers are with respect 40 to tris. 0.01 M tris/phosphate pH 8.5 (to wash venom onto the column) (fractions 1, 2, 3). 0.01 M tris/phosphate pH 7.0 (fraction 4), 0.02 M tris/phosphate pH 6.0, 0.04 M tris/phosphate pH 6.0 (fraction 5), 0.10 M tris/phosphate pH 6.0 (fraction 6), 0.10 M tris/phosphate + 0.10 M NaCl pH 6.0 (fraction 7). The changes in eluting buffer are made after the column has equilibrated with the buffer. The protein fractions obtained in this way are assayed for coagulant activity. Less than 1% of the applied coagulant activity is recovered in fraction Nos. 1, 2, 3, 4. Fraction 1 however possesses proteolytic activtity which in concentrated solutions would dissolve fibrin clots. The thrombin-like activity is eluted from these columns in significant amounts at a buffer strength of 0.04 M or greater. The eluate is freeze dried to give a light powdery product. Yield: 18-20 mg per 350 mg dry venom. Calculation of molecular weight of the protein based on the amino acid composition gives a value of 30.000 which is in a very good agreement with that obtained by physical methods.

Brand name

Venacil (Abbott).