9047-22-7

Basic information

• Product Name: EC 3.4.22.1
• Synonyms: CATHEPSIN B;CATHEPSIN B1, BOVINE SPLEEN;CATHEPSIN B, BOVINE SPLEEN;EC 3.4.22.1;CATHEPSIN B FROM HUMAN LIVER;CATHEPSIN B FROM HUMAN PLACENTA;CATHEPSIN B FROM BOVINE SPLEEN;CathepsinB from human placenta,CathepsinB1
• CAS NO: 9047-22-7
• Molecular Formula: NULL
• Molecular Weight: 0
• Mol File: 9047-22-7.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: −20°C
• Solubility: H2O: soluble1.0mg/mL, clear
• CAS DataBase Reference: EC 3.4.22.1(CAS DataBase Reference)
• NIST Chemistry Reference: EC 3.4.22.1(9047-22-7)
• EPA Substance Registry System: EC 3.4.22.1(9047-22-7)

Safety Data

• Hazard Codes: B
• WGK Germany: 3
• RTECS: FI5725000
• F: 10-21
• Hazardous Substances Data: 9047-22-7(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 9047-22-7 differently. You can refer to the following data:
1. Cathepsin B from human liver has been used:as a positive control in the screening Cathepsin B inhibitor assayin wall extension (expansin activity) assayin microinjection human foreskin fibroblasts to test its effect on apoptosis induction
2. Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer′s research, as it may have a role in the natural defense against the disease . Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells.

General Description

Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.

Biochem/physiol Actions

Cathepsin B displays an endopeptidase and peptidyl dipeptidase activities. It may be associated with the pathophysiology of tumors. Cathepsin B is also regarded as a prominent protease in Leishmaniasis. It is overactivated in muscular dystrophy, pulmonary emphysema, and bone resorption.

Purification Methods

Cathepsin B is purified by affinity chromatography on the semicarbazone of Gly-Phe-glycinal-linked to Sepharose 4B, with elution by 2,2'-dipyridyl disulfide [Rich et al. Biochem J 235 731 1986, Methods Enzymol 80 551 1981].