9074-87-7

Basic information

• Product Name: CARBOXYPEPTIDASE G
• Synonyms: GAMMA-GLUTAMYL HYDROLASE;EC 3.4.19.9;CARBOXYPEPTIDASE G;carboxypeptidase G from pseudomonas sp. purificat;Hydrolase, gamma-glutamyl;CARBOXYPEPTIDASE G FROM PSEUDOMONAS SP.;γ-glutamyl hydrolase;Glucarpidase
• CAS NO: 9074-87-7
• Molecular Formula: Zn4+8
• Molecular Weight: 261.56
• EINECS: 232-978-1
• Mol File: 9074-87-7.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: −20°C
• Solubility: H2O: soluble0.5mg/mL
• CAS DataBase Reference: CARBOXYPEPTIDASE G(CAS DataBase Reference)
• NIST Chemistry Reference: CARBOXYPEPTIDASE G(9074-87-7)
• EPA Substance Registry System: CARBOXYPEPTIDASE G(9074-87-7)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 9074-87-7(Hazardous Substances Data)

Usage

Description

Carboxypeptidase G is an enzyme derived from Pseudomonas sp., also known as γ-Glutamyl hydrolase. It plays a significant role in various biological processes and has been extensively studied for its potential applications in different fields.

Uses

Used in Pharmaceutical Industry:
Carboxypeptidase G is used as a research tool for studying the role of putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. This enzyme helps in understanding the mechanisms of drug metabolism and the development of new pharmaceutical compounds.
Used in Cancer Research:
Carboxypeptidase G is used as a research enzyme to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO?cGMP signaling in lung adenocarcinoma cells. This application aids in the study of cancer cell behavior and the development of targeted therapies for lung adenocarcinoma.

Biochem/physiol Actions

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.