9075-65-4 Usage
Description
EC 1.1.1.8, also known as α-glycerophosphate dehydrogenase, is an enzyme that catalyzes the reversible oxidation of sn-glycerol 3-phosphate to dihydroxyacetone phosphate. It plays a crucial role in various metabolic pathways, including glycolysis and gluconeogenesis.
Uses
Used in Biochemistry Research:
EC 1.1.1.8 is used as a research tool for studying enzymatic reactions and metabolic pathways. It is particularly useful in assays involving the cleavage of 2-deoxy-ribose 5-phosphate aldolase (DERA) and the measurement of glycerol kinase activity.
Used in Enzyme Assays:
α-Glycerophosphate Dehydrogenase from rabbit muscle is used in enzyme assays to demonstrate compartmentalized enzymatic reactions where NADH is involved. This helps researchers understand the spatial organization and regulation of metabolic pathways.
Used in L-fuculose-1-phosphate Aldolase Assay:
EC 1.1.1.8 is also used in the reaction mixture assay for L-fuculose-1-phosphate aldolase, an enzyme involved in the biosynthesis of fucose, a component of various glycoconjugates. This application aids in the study of carbohydrate metabolism and the role of aldolases in sugar biosynthesis.
Biochem/physiol Actions
α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.
Purification Methods
The dehydrogenase is recrystallised by adding (NH4)2SO4 to 0.45 saturation at pH 5.5 at 4oC, and the small amount of precipitate is removed, then a saturated solution of (NH4)2SO4 is added dropwise from time to time over several days in the cold room. The crystals are collected and recrystallised until they have maximum activity. The enzyme is stable in half-saturated (NH4)2SO4 for several weeks at 4o. The equilibrium [dihydroxyacetone][NADH][H+]/[G-3-P][NAD] is 1.0 x 10 -12M in Tris buffer at 25o. It uses NAD ten times more efficiently than NADP. The Km for G-3-P is 1.1 x 10 -4M, for NAD it is 3.8 x 10-4M and for dihydroxyacetone it is 4.6 x 10-4M in phosphate buffer pH 7.0 and at 23.3o. Dihydroxyacetone phosphate and fructose-1,6-diphosphate are inhibitors. [Branowski J Biol Chem 180 515 1949, The Enzymes 7 85 1963, Young & Pace Arch Biochem Biophys 75 125 1958, Walsh & Sallach Biochemistry 4 1076 1965.]
Check Digit Verification of cas no
The CAS Registry Mumber 9075-65-4 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,7 and 5 respectively; the second part has 2 digits, 6 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 9075-65:
(6*9)+(5*0)+(4*7)+(3*5)+(2*6)+(1*5)=114
114 % 10 = 4
So 9075-65-4 is a valid CAS Registry Number.
