92-32-0 Usage
Uses
Different sources of media describe the Uses of 92-32-0 differently. You can refer to the following data:
1. Bacterial and biological stain.
2. Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.
3. Pyronin Y has been used: in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)staining of CD34+ cells
Definition
ChEBI: An organic chloride salt having 6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium as the cation. Used with methyl green to selectively demonstrate RNA (red) in contrast to DNA (green) with the Unna
Pappenheim method.
General Description
Pyronin Y (C.I. 45005) is an aminoxanthene dye that is present as a weakly lipophilic cation in neutral aqueous solutions. It is also known as pyronine G.
Biochem/physiol Actions
Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.
Purification Methods
Commercial material may contain a large quantity of zinc. Purify it by dissolving 1g in 50mL of hot water containing 5g NaEDTA. Cool to 0o, filter, evaporate to dryness and the residue is extracted with EtOH. The solution is evaporated to 5-10mL, filtered, and the dye is precipitated by addition of excess of dry diethyl ether. It is centrifuged, and the crystals are washed with dry ether. The procedure is repeated, then the product is dissolved in CHCl3, filtered and evaporated. The dye is stored in a vacuum. [Beilstein 18 H 596, 18 III/IV 7361, 18/10 V 181.]
Check Digit Verification of cas no
The CAS Registry Mumber 92-32-0 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 9 and 2 respectively; the second part has 2 digits, 3 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 92-32:
(4*9)+(3*2)+(2*3)+(1*2)=50
50 % 10 = 0
So 92-32-0 is a valid CAS Registry Number.
InChI:InChI=1/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
92-32-0Relevant articles and documents
Supramolecular chemistry of pyronines B and Y, β-cyclodextrin and linked β-cyclodextrin dimers
Ngo, Huy T.,Clements, Philip,Easton, Christopher J.,Pham, Duc-Truc,Lincoln, Stephen F.
experimental part, p. 687 - 692 (2010/08/22)
The complexation of cationic pyronine B(PB+) and pyronine Y(PY+) by β-cyclodextrin (CD) and two linked βCD dimers, N,N′-bis((2AS,3AS)-3A-deoxy-β- cyclodextrin-3A-yl)succinamide, 33βCD2
