94827-27-7Relevant articles and documents
Syntheses of cellotriose and cellotetraose analogues as transition state mimics for mechanistic studies of cellulases
Noguchi, Shogo,Takemoto, Shintaro,Kidokoro, Shun-Ichi,Yamamoto, Kazunori,Hashimoto, Masaru
experimental part, p. 3812 - 3830 (2011/08/02)
Cellotriose and cellotetraose analogues carrying cyclohexene rings were developed as molecular probes which are expected to mimic the transition state conformation of hydrolysis by cellulases. The cyclohexene ring was placed at the pyranose ring being expected to locate the -1 subsite of the enzyme. In order to evaluate these probes, sulfur derivatives of cellotriose and cellotetraose were also synthesized as the enzyme tolerant analogues which mimic the stable conformations of the natural cellulose. The binding assays using differential scanning calorimetry revealed that introduction of the cyclohexene ring is effective to the complexation with an endoglucanase, NCE5 from Humicola insolens.