99792-79-7

Basic information

• Product Name: CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID
• Synonyms: CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID;CHLOROPHENOL RED BETA-D-GALACTOPYRANOSIDE (CPRG);Chlorophenolred-b-D-galactopyranoside;chlorophenol red galactopyranoside;Chlorophenol Red-β-D-galactopyranoside ,90%;beta-D-Galactopyranoside 2-chloro-4-[3-(3-chloro-4-hydroxyphenyl)-1,1-dioxido-3H-2,1-benzoxathiol-3-yl]phenyl;Chlorophenol Red-β-D-galactoside
• CAS NO: 99792-79-7
• Molecular Formula: C₂₅H₂₂Cl₂O₁₀S
• Molecular Weight: 585.40718
• EINECS: 1592732-453-0
• Mol File: 99792-79-7.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 791.4 °C at 760 mmHg
• Flash Point: 432.4 °C
• Appearance: /
• Density: 1.661
• Vapor Pressure: 2.13E-26mmHg at 25°C
• Refractive Index: 1.697
• Storage Temp.: −20°C
• CAS DataBase Reference: CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID(CAS DataBase Reference)
• NIST Chemistry Reference: CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID(99792-79-7)
• EPA Substance Registry System: CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID(99792-79-7)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 99792-79-7(Hazardous Substances Data)

Usage

Description

Chlorophenol red β-D-galactopyranoside (CPRG) is a colorimetric substrate for β-galactosidase. Upon enzymatic cleavage by β-galactosidase, chlorophenol red is released, which can be quantified by colorimetric detection at 570 nm as a measure of β-galactosidase activity.

Uses

Different sources of media describe the Uses of 99792-79-7 differently. You can refer to the following data:
1. Chlorophenol Red-β-D-galactopyranoside measures β-galactosidase expression levels in transfected cells. Chlorophenol Red-β-D-galactopyranoside is a substrate of β-Galactosidase.
2. Chlorophenol Red-β-D-galactopyranoside has been used as a substrate for β-galactosidase (β-gal):in paper-based cell-free protein expression system for target RNA detectionin colorimetric detection of multiple clinically relevant mutations using a lacZ output genein colorimetric assay for bacteria quantification
3. Colorimetric determination of β-Gal activity in cell extracts and ELISA.

General Description

Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength yellow dye commonly used for colorimetric assays. It serves as a substrate for β-galactosidase (β-gal) which hydrolyzes CPRG to form chlorophenol red (purple colored). This product is quantified by measuring its absorbance at 570 nm. CPRG is used to identify fecal coliform bacteria. It is about 10 times more sensitive than other chromogenic agents such as o-nitrophenyl-β-galactoside (ONPG). CPRG also has a high rapid turnover.

Biochem/physiol Actions

Chlorophenol red-β-D-galactopyranoside (CPRG) might possess oestrogenic activity.

InChI:InChI=1/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)25(14-3-1-2-4-20(14)38(33,34)37-25)13-6-8-18(16(27)10-13)35-24-23(32)22(31)21(30)19(11-28)36-24/h1-10,19,21-24,28-32H,11H2/t19-,21+,22+,23-,24-,25?/m1/s1

Synthetic route

chlorophenol red mono-β-D-galactopyranoside (99792-79-7) → chlorophenol red

Conditions	Yield
With β-galactosidase at 25℃; pH=7.4; aq. phosphate buffer;