Vitamin D2 USP USP Food Additive made in china CAS NO.50-14-6

properties

should be a white needle-shape crystal or white crystallized powder;give out no smell;no taste;be easy to lose activity in air or light

identification

1、dissolve about 0.5mg of vitamin d2 into 5ml chloroform, add 0.3ml acetic anhydride and 0.1ml sulfuric acid, shake and mix vigorously.the mixture is initially produced bright red color, and then rapidly changes violet and finally blue to green.

2、the infrared absorption spectroscopy should be in according with spectrum of comparison

examination

dissolve 10mg of vitamin d2 into 2ml of 90% ethanol, add 2ml solution of digitalis saponin and incubate for 18 hours. no precipitation or cloud should be observed

melting range

115℃~119℃

specific rotation

+103°~+106°

reducing substance

light absorption of sample

ergosterol

none

solubility

freely soluble in alcohol

organic volatile impurities

benzene ≤ 2ppm

content

97%~103%

total microbacterial count

≤1000cfu/g

total yeast & mould

≤100cfu/g

escherichia coli presence

absent