Evidence that the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate (cas 104302-77-4) is a product of the methylreductase reaction in Methanobacterium
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Add time:08/03/2019 Source:sciencedirect.com
When 7-mercaptoheptanoylthreonine phosphate (cas 104302-77-4) (HS-HTP) was used as the sole source of electrons for reductive demethylation of 2-(methylthio)-ethanesulfonic acid (CH3-S-CoM) by cell extracts of Methanobacterium thermoautotrophicum strain ΔH, the heterodisulfide of coenzyme M and HS-HTP (CoM-S-S-HTP) was quantitatively produced: HS-HTP + CH3-S-CoM → CH4 + CoM-S-S-HTP. CH4 and CoM-S-S-HTP were produced stoichiometrically in a ratio of 1:1. Coenzyme M (HS-CoM) inhibited HS-HTP driven methanogenesis indicating that CH3-S-CoM rather than HS-CoM was the substrate for CoM-S-S-HTP formation.
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