Glucose transfer from dolichol monophosphate (cas 12698-55-4) glucose The lipid moiety of the endogenous microsomal acceptor

Add time:08/28/2019    Source:sciencedirect.com

Further studies on the structure of the microsomal endogenous substance which accepts labeled glucose from dolichol monophosphate (cas 12698-55-4) glucose are reported. In order to obtain sufficient amounts of substance for measuring bound dolichol monophosphate, a concentrate was prepared by treating liver with mixtures of chloroform-methanol-water which lead to purification of the radioactive compound.Estimation of dolichol monophosphate, by its accelerating action on dolichol monophosphate glucose formation, showed that it is liberated by mild acid treatment of the concentrate. The dolichol monophosphate-yielding substance behaved like the labeled glucosylated acceptor when tested by its solubility properties in some solvent mixtures, thin-layer and DEAE-cellulose chromatographies and acid or alkaline treatments. Therefore the results provide further evidence that the lipid moiety of the endogenous acceptor of microsomes is dolichol phosphate.

We also recommend Trading Suppliers and Manufacturers of dolichol monophosphate (cas 12698-55-4). Pls Click Website Link as below: cas 12698-55-4 suppliers

Prev:ReviewHypoglycosylation due to dolichol metabolism defects
Next:Monoclonal antibody to amphomycin. A tool to study the topography of dolichol monophosphate (cas 12698-55-4) in the membrane)