Spermidine (cas 124-20-9) ameliorates the neuronal aging by improving the mitochondrial function in vitro
-
Add time:09/25/2019 Source:sciencedirect.com
Changes in mitochondrial structure and function are the initial factors of cell aging. Spermidine (cas 124-20-9) has an antiaging effect, but its effect on neuronal aging and mitochondrial mechanisms is unclear. In this study, mouse neuroblastoma (N2a) cells were treated with d‑galactose (d‑Gal) to establish cell aging to investigate the antiaging effect and mechanisms of spermidine. Changes in the cell cycle and β-galactosidase activity were analyzed to evaluate the extent of cell aging. Stabilities of mitochondrial mRNA and mitochondrial membrane potential (MMP) were evaluated in the process of cell aging under different treatments. The mitochondrial function was also evaluated using the Seahorse Metabolic Analysis System combined with ATP production. The unfolded protein response (UPR) of the N2a cells was analyzed under different treatments. Results showed that spermidine pretreatment could delay the cell aging and could maintain the mitochondrial stability during d‑Gal treatment. Spermidine increased the proportion of cells in the S phase and maintained the MMP. The oxygen utilization and ATP production in the N2a cells were reduced by d‑Gal treatment but were partially rescued by the spermidine pretreatment. Spermidine ameliorated the N2a cell aging by promoting the autophagy and inhibiting the apoptosis except the UPR. These results showed that spermidine could ameliorate the N2a cell aging by maintaining the mitochondrial mRNA transcription, MMP and oxygen utilization during the d‑Gal treatment.
We also recommend Trading Suppliers and Manufacturers of Spermidine (cas 124-20-9). Pls Click Website Link as below: cas 124-20-9 suppliers
Prev:Spermidine (cas 124-20-9) induced aggregation of terphenyl derivative: An efficient probe for detection of Spermidine (cas 124-20-9) in living cells
Next:Polyamine oxidase 2 is involved in regulating excess Spermidine (cas 124-20-9) contents during seed germination and early seedling development in Arabidopsis thaliana) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Corrosion inhibition performance of Spermidine (cas 124-20-9) on mild steel in acid media09/28/2019
- Effect of Spermidine (cas 124-20-9) and D-arginine treatments on ethylene synthesis in Xinjiang ‘Saimaiti’ apricot10/01/2019
- Spermidine (cas 124-20-9) influences enhanced micropropagation and antibacterial activity in Aerva javanica (Burm. F.) Shult.09/27/2019
- Polyamine oxidase 2 is involved in regulating excess Spermidine (cas 124-20-9) contents during seed germination and early seedling development in Arabidopsis thaliana09/26/2019
- Spermidine (cas 124-20-9) induced aggregation of terphenyl derivative: An efficient probe for detection of Spermidine (cas 124-20-9) in living cells09/24/2019
- Helicobacter pylori does not use Spermidine (cas 124-20-9) synthase to produce Spermidine (cas 124-20-9)09/10/2019
- Cardioprotection by Spermidine (cas 124-20-9) does not depend on structural characteristics of the myocardial microcirculation in aged mice09/09/2019
- Spectroscopic and molecular docking studies on the interaction between Spermidine (cas 124-20-9) and pancreatic elastase09/08/2019