Simultaneous determination of malachite green and its metabolite LEUCOMALACHITE GREEN (cas 129-73-7) in eel plasma using post-column oxidation
-
Add time:09/27/2019 Source:infona.pl
A rapid HPLC method with solid-phase extraction (SPE) clean-up for malachite green (MG) and LEUCOMALACHITE GREEN (cas 129-73-7) (LMG) in eel plasma was developed. MG and LMG were extracted with a buffered methanolic solution. The extract was subjected to aromatic sulphonic acid SPE. MG and LMG were eluted from the SPE column with methanol after a treatment with ammonia gas. The reconstituted eluate was analyzed on a Chromspher B column with acetonitrile-ion-pair buffer (pH 4.0) (6:4, v/v) as the mobile phase and detection at 610 nm after post-column oxidation with PbO 2 . The average recoveries for MG and LMG over the linear range of applicability (20-2500 ng/ml) were 82 ± 1% and 83 ± 1%, respectively. The limits of quantification were 5.0 μg/l for MG and 0.9 μg/l for LMG.
We also recommend Trading Suppliers and Manufacturers of LEUCOMALACHITE GREEN (cas 129-73-7). Pls Click Website Link as below: cas 129-73-7 suppliers
Prev:Simultaneous and Fast Determination of Malachite Green, LEUCOMALACHITE GREEN (cas 129-73-7), Crystal Violet, and Brilliant Green in Seafood by Ultrahigh Performance Liquid Chromatography–Tandem Mass Spectrometry
Next:1H NMR and computational studies of the conformations in solution of one host/guest complex formed with an usnic acid tweezer and 2,4,7-TRINITRO-9-FLUORENONE (cas 129-79-3) (TNF)) - 【Back】【Close 】【Print】【Add to favorite 】