- Chemoenzymatic synthesis of L-3,4-dimethoxyphenyl-alanine and its analogues using aspartate aminotransferase as a key catalyst
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In this study, a chemoenzymatic synthesis method for the production of L-3,4-dimethoxyphenyl-alanine and its analogues from phenylpyruvate derivatives was developed. The aspartate aminotransferase from Escherichia coli was engineered by error prone PCR and the improved variants were identified. When 3, 4-dimethoxy phenylpyruvate was added by fed-batch on a preparative scale, L-3,4-dimethoxyphenyl-alanine was formed in 95.4% conversion and > 99% ee with the best aspartate aminotransferase variant as the catalyst. This study provided an efficient method for the production of methoxy substituted phenylalanines using the engineered aspartate aminotransferase.
- Yu, Jinhai,Li, Jing,Cao, Shuangyan,Wu, Ting,Zeng, Shuiyun,Zhang, Hongjuan,Liu, Junzhong,Jiao, Qingcai
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- Expedient base-mediated desulfitative dimethylamination, oxidation, or etherification of 2-(Methylsulfanyl)-3,5-dihydro-4 H -imidazol-4-one scaffolds
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The dimethylamino functionality is generally introduced onto the 3,5-dihydro-4H-imidazol-4-one skeleton by treatment of a halogenated derivative with low-boiling dimethylamine at a high temperature and pressure. The corresponding aliphatic ethers are usua
- Khan, Shahnawaz,Tyagi, Vikas,Mahar, Rohit,Bajpai, Vikas,Kumar, Brijesh,Chauhan, Prem M.S.
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p. 2405 - 2412
(2013/09/23)
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- Antiviral 5-(substituted benzal) hydantoins
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Therapeutic compositions containing a compound of the formula: STR1 wherein: R1, R2, and R3 are each hydrogen, hydroxy, alkoxyf 1 to 4 carbon atoms, acyloxy of 1 to 4 carbon atoms, halo, or nitro, or R2 and Rsu
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