- A biological buffer preparation method
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The invention discloses a preparation method for a biological buffer agent which has a general reaction formula shown in the description. In the general reaction formula, n is equal to 1, 2, 3, or 4, and M is K or Na. The synthesis process of the biological buffer agent has the main advantages of being low in solvent consumption, little in sewage, high in reaction efficiency, and easy for industrialization; in the synthesis process, raw materials are simple and easy to obtain; compared with the conventional preparation method that diethanol amine and butene sodium sulfonate or chloroethanesulfonic acid are taken as raw materials for reaction, the preparation method provided by the invention achieves a breakthrough in the synthesis process, that is, a compound III is obtained through reaction between ethylene epoxide and sulfamate, so that the reaction yield and efficiency are greatly improved, and the problem of low reaction yield and efficiency in the prior art is solved.
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Paragraph 0069; 0070
(2017/08/02)
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- A new strategy for the synthesis of taurine derivatives using the 'safety-catch' principle for the protection of sulfonic acids
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The safety-catch principle has been applied for the development of a new method for protecting sulfonic acids. 2,2-Dimethylsuccinic acid was reduced to 2,2-dimethylbutane-1,4-diol, which was selectively silylated to give 4-(tert-butyldiphenylsilanyloxy)-2,2-dimethylbutan-1-ol. Reaction of the latter compound with 2-chloroethanesulfonyl chloride in the presence of triethylamine afforded 4-(tert-butyldiphenylsilyloxy)-2,2-dimethylbutyl ethenesulfonate directly. The ethenesulfonate underwent Michael-type addition with secondary amines to give protected derivatives of taurine (2-aminoethanesulfonic acid). Deprotection was achieved on treatment with tetrabutylammonium fluoride, whereby cleavage of the silicon-oxygen bond led to an intermediate alkoxide that immediately cyclised to 2,2-dimethyltetrahydrofuran with liberation of a sulfonate. Pure sulfonic acids were obtained from the crude product by ion exchange chromatography on a strongly basic resin, which was eluted with aqueous acetic acid. The method developed should be generally applicable to the protection of sulfonic acids and is amenable to a multiparallel format. This journal is The Royal Society of Chemistry.
- Seeberger, Sonja,Griffin, Roger J.,Hardcastle, Ian R.,Golding, Bernard T.
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p. 132 - 138
(2008/03/14)
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- Isolation of nucleic acids
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A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.
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- Isolation of nucleic acids
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A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.
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- Zwitterionic compounds and their n-halo derivatives for use in the treatment of clinical conditions
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Zwitterionic compounds selected from: taurine (2-aminoethanesulphonic acid), 2(N-morpholino)ethanesulphonic acid (MES), N-(2-acetamido)iminodiacetic acid (ADA), piperazine-N,N'bis(2-ethanesulphonic acid (PIPES), N-(2-acetamido)-2-aminoethanesulphonic acid (ACES), N,N-bis(2-hydroxyethyl)-2-aminoethanesulphonic acid (BES), 3-(N-morpholino)propanesulphonic (MOPS), N-N[tris(hydroxymethyl)-methyl]-2-aminoethanesulphonic acid (TES), N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid (HEPES), N-2-hydroxyethylpiperazine-N'3-propanesulphonic acid (H)EPPS), 2-(cyclohexylamino)ethanesulphonic acid (CHES) or 3-(cyclohexylamino)propanesulphonic acid (CAPS), and their N-halo derivatives can be used separately or in combination in the treatment of related clinical conditions by stimulating myeloperoxidase activity, which in turn stimulates hypochlorous acid production in vivo, which leads inter alia to enhanced leukotriene inactivation.
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- Pharmaceutical composition and method of treating psoriasis
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A method of treating psoriasis and similar or related ailments comprises applying to an affected skin area a therapeutically effective amount of at least one skin-compatible zwitterionic aminosulfonic acid (ZASA) of the formula wherein R is a straight or branched chain aliphatic radical, or RN is a substituted or unsubstituted nitrogen-containing heterocycle which may have one additional hetero atom; and R' is C2-4 straight or branched chain alkylene radical. A pharmaceutical composition is also provided, and comprises at least one ZASA of the above formula together with a pharmaceutically acceptable topical carrier or base.
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- Novel taurine derivatives
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Novel taurine derivatives of general formula (I) STR1 (wherein R1 is nicotinoyl, 3,4,5-trimethoxybenzoyl or acetylsalicyloyl and R2 is as defined for R1 or is --CH2 CH2 OR1) have antilipemic and choleretic activities. The compound in which R2 =--CH2 CH2 OR1 and R1 is acetylsalicyloyl exhibits high antiinflammatory, analgesic and antipyretic activity and is essentially free of the unfavorable effect of acetylsalicylic acid on the stomach.
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