A FACILE ONE-STEP SYNTHESIS OF 5'-PHOSPHATIDYLNUCLEOSIDES BY AN ENZYMATIC TWO-PHASE REACTION.
Phospholipase D from Streptomyces effectively catalyzed the transfer reaction of the phosphatidyl residue from phosphatidylcholines to the 5'-hydroxyl group of nucleosides in a two-phase system.A variety of 5'-phosphatidylnucleosides were easily prepared in high yields by this reaction.
