Solution-phase synthesis of branched DNA hybrids via H -phosphonate dimers
A method for the solution-phase synthesis of branched oligonucleotides with tetrahedral or pseudo-octahedral geometry is described that involves the coupling of 3′-H-phosphonates of protected dinucleoside phosphates and organic core molecules. The dimer building blocks are produced by a synthesis that requires no chromatographic purification and that produces the dimer H-phosphonates in up to 44% yield in less than three days of laboratory work. A total of seven different branched hybrids were prepared, including a new hybrid of the sequence (CG)4TBA, where TBA stands for tetrakis(p- hydroxybiphenyl)adamantane that assembles into a material from micromolar aqueous solution upon addition of MgCl2.
Singh, Arunoday,Tolev, Mariyan,Schilling, Christine I.,Braese, Stefan,Griesser, Helmut,Richert, Clemens
experimental part
p. 2718 - 2728
(2012/06/30)
Use of ammonium aryl H-phosphonates in the preparation of nucleoside H-phosphonate building blocks
Ammonium 4-methylphenyl H-phosphonate 4c was used in the conversion of 5'-0-(dimethoxy-trityl)-2'-deoxynucleoside derivatives 6 into the corresponding 3'-H-phosphonates 2, which were isolated in a high state of purity and in high yield.
Ozola, Vita,Reese, Colin B.,Song, Quanlai
p. 8621 - 8624
(2007/10/03)
NUCLEOSIDE H-PHOSPHONATES. III. CHEMICAL SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES BY THE HYDROGENPHOSPHONATE APPROACH
A rapid synthesis of oligodeoxyribonucleotides on solid support is described via coupling of the deoxyribonucleoside 3'-H-phosphonates in the presence of various condensing reagents.
Garegg, Per J.,Lindh, Ingvar,Regberg, Tor,Stawinski, Jacek,Stroemberg, Roger,Henrichson, Christina
p. 4051 - 4054
(2007/10/02)
More Articles about upstream products of 110237-98-4